食管上皮癌变过程中DNA 含量及细胞周期调控因子表达的定量检测

 目的 探讨食管上皮癌变过程中DNA含量、细胞周期分布的变化及多个相关的细胞周期调控因子的表达状况及其意义. 方法 应用碘化丙啶染色和间接免疫荧光标记方法,采用流式细胞仪对30例食管癌组织及相应的癌旁组织进行定量检测. 结果 与正常黏膜和非典型增生组织相比,癌组织中DNA含量明显增高,异倍体细胞显著增加;G0/G1期细胞明显减少,而S期和G2/M期细胞显著增多,增殖指数（PI）高于癌旁组织;cyclinE、cdk2、p53、E2F基因蛋白过表达,两两之间皆有显著性正相关关系,抑癌基因p21^WAF1基因蛋白表达水平明显降低,与其余基因蛋白之间有明显的负相关. 结论 食管上皮癌变过程中,DNA含量及异倍体率增加,细胞周期分布发生了明显的改变,细胞周期相关基因蛋白表达异常.

Quantities Detection of DNA Content and Expression of Cell Cycle Regulatory Elements in Esophageal Canceration Course

Objective 　To explore the change of DNA content , cell cycle distribution and expression of cell cycle regulatory elements in esophageal canceration course. Methods 　30 cases of esophageal carcinoma and corresponding adjacent noncancerous tissue were detected quantitatively by propidium iodide ( PI) stain and indirect immunofluorescence of fluorescein isothiocyanate ( FITC) using flow cytometry ( FCM) equipment . Results 　Comparing with normal epithelium and dysplasia tissue , DNA content and heteroploid cells increased significantly in esophageal carcinoma. The percentage of cells in G₀ / G₁ phase decreased while which increased in G₂ / M phase sharply , the proliferation index ( PI) enhanced in carcinoma than in corresponding adjacent noncancerous tissue. Expression of cyclinE、cdk2 、p53 、E₂ F proteins increased obviously while p21^WAF1 expression decreased in esophageal carcinoma. There are significant positive correlations among the expressions of cyclinE、cdk2 、p53 、E₂ F proteins each other , but there is a negative correlation between p21^WAF1 protein and each of them. Conclusion 　During the esophageal canceration course , DNA content and heteroploid rate increased significantly. Cell cycle dist ribution changed obviously. Expressions of relevant cell cycle regulatory element s were abnormal.