麦冬不同种群遗传多样性的RSAP分析

利用RSAP标记技术对120份不同地理来源的麦冬种质资源进行多样性分析,结果表明:筛选出的16对引物组合共扩增出326条带,其中318条多态性条带,多态性比率为97.55%,多态性含量PIC在0.87~0.95,平均0.92,表明试供材料间在分子水平上具有丰富的遗传多样性。种群遗传多样性结果表明,20个种群多态性位点比例(PPL)为19.94%~85.58%,Nei’s基因多样性指数(H)和Shannon信息指数(I)分别在0.082 6~0.210 7,0.120 6~0.328 1,其中浙麦冬遗传多样性最高,矮小山麦冬遗传多样性最低。种群遗传距离在0.024 6~0.286 8,浙江山麦冬和浙江阔叶山麦冬遗传距离最小,福建短葶山麦冬和沿阶草遗传距离最大。20个自然种群间基因分化系数(Gst)为0.430 7,种群间遗传变异占总遗传变异的43.07%,种群内变异为56.93%,基因流(Nm)为0.660 9。UPMGA聚类分析与主坐标分析结果基本一致,120份样本被分成四大类,聚类结果与形态分类基本一致,同时与地理来源也有很高的的一致性。

Genetic diversity of different populations of lilyturf revealed by RSAP analysis

Restriction site amplification polymorphism (RSAP) markers were employed to access the genetic diversity and relationship of 120 lilyturf germplasms from different geographical origins. Sixteen RSAP primer pairs generated 326 polymorphic bands,of which 318(97.55%) were polymorphic. The value of polymorphism information content (PIC) ranged from 0.87 to 0.95 with an average of 0.92. These results indicated there was abundant genetic diversity among samples. The results of data analysis on 20 population showed that the value of percentage of polymorphic locus(PPL), Nei's gene diversity(H) and Shannon's information index(I) were 19.94%-85.58%, 0.082 6-0.210 7, 0.120 6-0.328 1 respectively. The most abundant genetic diversity was found in the O. japonicus population from Zhejiang and the least in the Liriope minor population. The genetic distance among 20 population was 0.024 6-0.286 8, of which the minimum genetic distance was 0.024 6 between population 1 and population 13 while the maximum 0.286 8 between population 5 and population 15. Coefficient of genetic differentiation among natural populations was 0.115 3(Gst). And the gene differentiation contributed to 43.07% of the total genetic variation among populations and to 56.93% within populations. The total gene flow (N_m) was 0.660 9. UPMGA clustering analysis was basically similar to of the principle coordinate analysis(PCA). The 120 samples were classified into four major groups, which were basically corresponded with the genetic relationships based on morphological traits. The results of UPMGA and PCA were also consistent with geographical origins.