中西医临床专业形态实验改革对学生动手能力的影响

<正>人体解剖学、组织学与胚胎学及病理学,属于基础医学课程中的形态学学习范畴,近年来,由于基础课学时缩短,加大了这些基础课程的学习难度,众所周知,实践教学是医学院校整体教学工作重要的组成部分1-5]。随着教学改革的不断深入,笔者大胆实践,开展了以提高动手能力和实践创新能力为导向的实验课教学改革,将形态学课程中的实验教学部分进行整合,在原有课时不变的基础上,增加综合实验内容,充分提高学生的动手能力,并

The origin identification of Chinese traditional medicine Dahuang Zhechong pill based on metabarcoding method

Objective] To investigate the ability of metabarcoding technology as one kind of identification tool for the origin of Chinese traditional patent medicine. Methods] The DNA of TCMDahuang Zhechong pill was extracted by paramagnetic particle method, and the 16S, trnL and Mini-barcode fragments were amplified by real-time PCR. The products of PCR were cloned and sequenced. After BLASTn alignment, the sequences were parsed into taxonomic term by MEGAN. The sequences representing TCM Mengchong were analysed by MEGA. Results] The TCMQicao, Mengchong, licorice and Peach Seed & Bitter Apricot Seed were identified from 23 sequences. Three groups of individuals with significant genetic differences were identified from 6 sequences that representing TCM Mengchong. Conclusion] Metabarcoding technology could be used for component identification of Chinese traditional patent medicine and should be an useful tool for quality evaluation of Chinese traditional patent medicine.