| 碱裂解法快速提取中药炒制品DNA的研究 |
| |
| 引用本文： | 郑琪,蒋超,黄璐琦,张志杰,李娆娆,陈康,袁媛,金艳.碱裂解法快速提取中药炒制品DNA的研究[J].中国中药杂志,2014,39(19):3678-3683. |
| |
| 作者姓名： | 郑琪 蒋超 黄璐琦 张志杰 李娆娆 陈康 袁媛 金艳 |
| |
| 作者单位： | 陕西中医学院, 陕西 咸阳 712000;中国中医科学院 中药资源中心 道地药材国家重点实验室培育基地, 北京 100700;中国中医科学院 中药资源中心 道地药材国家重点实验室培育基地, 北京 100700;中国中医科学院 中药资源中心 道地药材国家重点实验室培育基地, 北京 100700;中国中医科学院 中药研究所, 北京 100700;中国中医科学院 中药研究所, 北京 100700;中国中医科学院 中药资源中心 道地药材国家重点实验室培育基地, 北京 100700;安徽中医药大学, 安徽 合肥 230038;中国中医科学院 中药资源中心 道地药材国家重点实验室培育基地, 北京 100700;中国中医科学院 中药资源中心 道地药材国家重点实验室培育基地, 北京 100700 |
| |
| 基金项目： | 中医药行业研究专项（201407003） |
| |
| 摘 要： | 该文旨在探索一种适用于中药炒制品DNA快速提取的方法。以氢氧化钠,1%PVP40和1%Triton X-100配制成碱裂解缓冲液,Tris-HCl为中和液,经加热裂解和中和2步提取不同炒制方法制备的中药炮制品的DNA,选择2种方法对DNA进行纯化,并以纯化后DNA作为模板利用通用引物进行PCR扩增。结果表明优化碱裂解法可简单快速的提取出药材DNA,槐米炒制品DNA质量浓度为(420.61±123.91)g·L-1,且使用5%Chelex-100树脂纯化可以提高DNA提取浓度。研究结果证明优化碱裂解法适用于中药炒制品的DNA快速提取。
|
| 关 键 词： | 中药炒制品 DNA快速提取 碱裂解法 Chelex-100 |
| 收稿时间： | 2014/3/24 0:00:00 |
Rapid extraction of DNA from Chinese medicinal products by alkaline lysis |
| |
| ZHENG Qi,JIANG Chao,HUANG Lu-qi,ZHANG Zhi-jie,LI Rao-rao,CHEN Kang,YUAN Yuan and JIN Yan.Rapid extraction of DNA from Chinese medicinal products by alkaline lysis[J].China Journal of Chinese Materia Medica,2014,39(19):3678-3683. |
| |
| Authors: | ZHENG Qi JIANG Chao HUANG Lu-qi ZHANG Zhi-jie LI Rao-rao CHEN Kang YUAN Yuan and JIN Yan |
| |
| Institution: | Shaanxi University of Chinese Medicine, Xianyang 712000, China;State Key Laboratory of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;State Key Laboratory of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;State Key Laboratory of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Institute of Chinese Materia Medica, Chinese Academy of Chinese Medical Sciences, Beijing 100700, China;Institute of Chinese Materia Medica, Chinese Academy of Chinese Medical Sciences, Beijing 100700, China;State Key Laboratory of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Anhui College of Traditional Chinese Medicine, Hefei 230038, China;State Key Laboratory of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;State Key Laboratory of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China |
| |
| Abstract: | The study is aimed to explore a rapid method to extract DNA from fried Chinese medicinal products. The alkaline lysis buffer was made of sodium hydroxide, 1%PVP and 1% TritonX-100 and Tris-HCl solution was neutralized, through heat cracking and neutralization two step to extract DNA from processed and prepared products of traditional Chinese medicine. Then universal primes were used to amplify PCR products for fired Chinese medicinal materials. The results indicated the optimized alkaline lysis method for extracting DNA is quick and easy. Extracting of the different processed Sophora japonica of DNA concentration was (420.61±123.91)g·L-1. Using 5% Chelex-100 resin purification can improve the DNA concentration. Our results showed that the optimized alkaline lysis method is suitable for Chinese medicinal materials for quickly DNA extraction. |
| |
| Keywords: | Chinese medicinal fried products quickly DNA extraction alkalinelysis Chelex-100 |
| 本文献已被 CNKI 等数据库收录！ |
|
