| 内生真菌Penicillium polonicum次生代谢产物研究 |
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| 引用本文： | 刘静,丁广治,方磊,庾石山. 内生真菌Penicillium polonicum次生代谢产物研究[J]. 中国中药杂志, 2014, 39(20): 3974-3977 |
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| 作者姓名： | 刘静 丁广治 方磊 庾石山 |
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| 作者单位： | 中国医学科学院 北京协和医学院药物研究所, 天然药物活性物质与功能 国家重点实验室, 北京 100050;中国医学科学院 北京协和医学院药物研究所, 天然药物活性物质与功能 国家重点实验室, 北京 100050;中国医学科学院 北京协和医学院药物研究所, 天然药物活性物质与功能 国家重点实验室, 北京 100050;中国医学科学院 北京协和医学院药物研究所, 天然药物活性物质与功能 国家重点实验室, 北京 100050 |
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| 基金项目： | 国家自然科学基金项目（21302226） |
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| 摘 要： | 选用PDB培养基发酵菌株,对内生真菌Penicillium polonicum次生代谢产物进行化学成分研究。结合运用凝胶Sephadex LH-20柱色谱、反相柱色谱和制备HPLC等多种色谱技术的方法从菌液的乙酸乙酯萃取物中分离鉴定了6个化合物,采用波谱学方法鉴定其结构分别为[3,5-二羟基-2-(7-羟基-辛酰)]-苯乙酸乙酯(1),(3,5-二羟基-2-辛酰)-苯乙酸乙酯(2),(5,7-二羟基-9-庚基)-异苯二氢吡喃-3-酮(3),3-(羟甲基)-4-(1E)-1-丙烯-1-基-(1R,2S,5R,6S)-7-氧双环[4.1.0]庚-3-烯-2,5-二醇(4),(E)-2-甲氧基-3-(1-丙烯基)苯酚(5),对羟基苯乙醇(6)。
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| 关 键 词： | PDB培养基 内生真菌Penicillium polonicum 次生代谢产物 |
| 收稿时间： | 2014-06-04 |
Study on secondary metabolites of endophytic fungi Penicillium polonicum |
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| LIU Jing,DING Guang-zhi,FANG Lei and YU Shi-shan. Study on secondary metabolites of endophytic fungi Penicillium polonicum[J]. China Journal of Chinese Materia Medica, 2014, 39(20): 3974-3977 |
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| Authors: | LIU Jing DING Guang-zhi FANG Lei YU Shi-shan |
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| Affiliation: | Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Beijing 100050, China;Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Beijing 100050, China;Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Beijing 100050, China;Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Beijing 100050, China |
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| Abstract: | The PDB culture medium was selected to ferment the endophyte strain, and the secondary metabolites of endophytic fungi Penicillium polonicum were studied. Combined application of Sephadex LH-20, ODS and HPLC chromatographies over the ethyl acetate extract of the fermented culture led to the isolation of 6 compounds. By spectral methods, the structures were elucidated as[3, 5-dihydroxy-2-(7-hydroxy-octanoyl)]-ethylphenylacetate (1), (3, 5-dihydroxy-2- octanoyl)-ethyl phenylacetate (2), (5, 7-dihydroxy-9-heptyl)-isobenzo pyran-3-one (3), 3-(hydroxymethyl)-4-(1E)-1- propen-1-yl-(1R, 2S, 5R, 6S)-7-oxabicyclo[4.1.0]hept-3-ene-2, 5-diol (4), (E)-2- methoxy-3-(prop-1-enyl) phenol (5) and p-hydroxylphenylethanol (6). |
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| Keywords: | PDB culture medium endophytic fungi Penicillium polonicum secondary metabolites |
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