拉应力对体外培养人足跟皮肤成纤维细胞增殖影响的实验研究

目的：观察拉应力对人足跟外倒皮肤成纤维细胞增殖的影响,为预防跟骨骨折术后切口边缘皮肤坏死、切口不愈合提供一定的理论依据.方法：取40岁男性足跟外侧皮肤成纤维细胞培养及传代后,分Oh组、6h组、24 h组进行拉应力加载试验,检测培养液中TGF-β1、IL-6含量,使用透射电镜、光镜观察线粒体、细胞核的变化.结果：在10％的拉应力下,成纤维细胞随着承受应力的时间增加,线粒体逐渐减少,TGF-β1和IL-6的分泌受到抑制,细胞增殖逐渐受到抑制.结论：人足跟外侧皮肤长时间处于拉应力状态是跟骨骨折手术后切口边缘皮肤坏死、切口不愈合的重要原因.

Effect of tensile stress on human heel skin fibroblast proliferation in vitro

Objective：To observe the effect of tensile stress on human heel skin fibroblast proliferation in vitro,providing a theoretical basis for preventing the wound edge skin necrosis and nonunion after calcaneal fracture surgery.Methods：Fibroblast cells were taken from lateral heel skin of a 40 year-old-man,then cultured and subcultured in vitro.After that,they were divided into three groups：0 hours group,6 hours group and 24 hours group and were tested by tensile stress testing.The levels of TGF-β1 and IL-6 in nutrient fluid were measured.Transmission electron microscope and light microscope was applied for observe mitochondria and nucleus.Results：Under 10％ of the tensile stress,mitochondria decreased,the levels of TGF-β1 and IL-6 in nutrient fluid were decreased and cell proliferation was inhibited gradually with time increasing.Conclusion：The human lateral heel skin in a long-time tensile stress state is an important cause of wound edge skin necrosis and nonunion after calcaneus fracture surgery.