不同寒热药性中药对酵母致热大鼠体温调节及温度敏感瞬时感受器电位离子通道（TRPs）蛋白的影响

目的:观察比较4种典型寒、热药性中药对酵母致大鼠发热模型体温的干预影响以及温度敏感瞬时感受器电位离子通道(TRPs)蛋白的调节作用。方法:背部皮下注射酵母混悬液建立大鼠发热模型,108只SD大鼠随机平均分为正常组、模型组、大黄组、黄连组、吴茱萸组和高良姜组,分别于造模后第4,8,12 h取下丘脑和背根神经节,通过免疫组化和Western blot方法检测下丘脑和背根神经节中温度敏感瞬时感受器电位离子通道蛋白TRPV1和TRPM8的水平。结果:与正常组相比,注射酵母后模型组大鼠体温明显上升,8 h达到高峰期(P<0.01);下丘脑和背根神经节中TRPV1水平显著升高,TRPM8水平则明显降低。与模型组比较,大黄和黄连可显著降低酵母引起的大鼠体温升高,下调下丘脑和背根神经节中TRPV1水平,上调TRPM8水平(P<0.05或P<0.01);吴茱萸和高良姜对发热大鼠的体温和TRPs均无明显影响。结论:寒性中药大黄和黄连能降低酵母致热大鼠的体温,其作用可能与其对下丘脑和背根神经节中TRPV1和TRPM8的有效调节有关;热性中药吴茱萸和高良姜无相关作用。

Effect of traditional Chinese medicines with different properties on thermoregulation and temperature-sensitive transient receptor potentialion channel protein of rats with yeast-induced fever

Objective: To compare the intervention effects of four traditional Chinese medicines(TCMs) with typical cold or hot property on body temperature and temperature-sensitive transient receptor potential ion channel proteins (TRPs) of rats with yeast-induced fever. Method: The pyrexia model was induced by injecting yeast suspension subcutaneously. Totally 108 male SD rats were randomly divided into the normal group, the model group, the Rhei Radix et Rhizoma treated group, the Coptidis Rhizoma treated group, the Euodiae Fructus treated group, and the Alpiniae Officinarum Rhizoma treated group, with 18 rats in each group. At the 4 h, 8 h and 12 h after injection of yeast, the rats were sacrificed to collect their hypothalamus and dorsal root ganglion. The expressions of TRPV1 and TRPM8 were detected by immunohistochemistry and Western blot method. Result: Compared with the normal group, after injection of yeast, the temperature of rats in the model group notably increased, and reached the peak at 8 h (P<0.01). The TRPV1 level in hypothalamus and dorsal root ganglia (DRG) of the model group significantly increased, whereas the TRPM8 level significantly reduced. Compared with the model group, the Rhei Radix et Rhizoma group and the Coptidis Rhizoma group showed significant decrease in the high body temperature of rats caused by yeast, down-regulation in the expression of TRPV1, and up-regulation in the expression of TRPM8 (P<0.05 or P<0.01). Euodiae Fructus and Alpiniae Officinarum Rhizoma had no significant effect on either temperature or TRPs of fever rats. Conclusion: Rhei Radix et Rhizoma and Coptidis Rhizoma, both are TCMs with cold property, can reduce the temperature of fever rats induced by yeast, which may be related to their effective regulation of TRPV1 and TRPM8 in hypothalamus and DRG, while Euodiae Fructus and Alpiniae Officinarum Rhizoma had no relevant effect.