UPLC法测定人参流动浸膏中8种人参皂苷类成分

目的]建立同时测定人参流动浸膏中8种人参皂苷类成分含量的超高效液相色谱法(UPLC)方法。方法]采用Waters AcqutityUPLC色谱系统,Acqutity UPLC誖BEH C18(1.7μm,2.1 mm×50 mm)色谱柱,流动相为乙腈-水,梯度洗脱,流速0.6 mL/min,柱温40℃,检测波长203nm。结果]人参皂苷Rg1、Re、Rf、Rb1、Rc、Rb2、Rb3和Rd分别在0.009 7~0.291 0μg,0.008 5~0.255 0μg,0.0075~0.300 0μg,0.010 4~0.416 0μg,0.010 7~0.428 0μg,0.007 4~0.296 0μg,0.004 2~0.168 0μg和0.009 1~0.364 0μg范围内成良好的线性关系,平均回收率分别为101.4%、101.1%、100.1%、99.8%、99.3%、98.8%、98.8%和100.5%。结论]方法快速、准确、重复性好,可为人参流动浸膏的质量控制提供快速准确的检测方法。

Determination of eight ginsenosides in Ginseng extract by UPLC

Objective] To develop a ultra-high performance liquid chromatography method for simultaneously determination of eight ginsenosides in Ginseng extract. Methods] The analysis was performed on a Waters Acqutity UPLC system eluted with mobile phases of aeetonitrile and water in gradient mode. The column was Acqutity UPLC BEH C18 （1.7 μm,2.1 mm×50 mm）. The flow rate was 0.6 mL/min. The column temperature was set at 40 ℃ and the detection wavelength was set at 203 nm. Results] Good linearity was obtained in the range of 0.009 7-0.291 0 μg, 0.008 5-0.255 0 μg, 0.007 5-0.300 0 μg, 0.010 4-0.416 0 μg, 0.010 7-0.428 0 μg, 0.007 4-0.296 0 μg, 0.004 2-0.168 0 μg and 0.009 1-0.364 0 μg for ginsenoside Rgl,Re,Rf,Rbl,Rc,Rb2,Rb3 and Rd respectively. The method recoveries of eight compounds were 101.4%, 101.1%, 100.1%, 99.8%, 99.3%, 98.8%, 98.8% and 100.5% respectively. Conclusion] The established method can rapidly receive an accurate and reproducible result used for controlling the quality of Ginseng extract.