Altered expression pattern of testican-1 mRNA after brain injury |
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Authors: | Iseki Ken Hagino Seita Zhang Yuxiang Mori Tetsuji Sato Nobuko Yokoya Sachihiko Hozumi Yasukazu Goto Kaoru Tase Choichiro |
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Affiliation: | Department of Emergency and Critical Care Medicine, Yamagata University, School of Medicine, Iida-Nishi 2-2-2, Yamagata City, Yamagata 990-9585, Japan. ken@med.id.yamagata-u.ac.jp |
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Abstract: | Testican, a chondroitin/heparan sulfate proteoglycan, is primarily expressed in neurons of the adult and embryonic mouse brain, suggesting its role in normal and/or proliferation and differentiation processes of neurons. However, the role of testican in injured brain remains unclear. In the present study we investigated testican-1 mRNA expression pattern after cryo-injury of the brain. In situ hybridization histochemistry revealed that testican-1 mRNA is induced in the region surrounding the necrotic tissue. Time course study of testican-1 mRNA showed the highest level of signal intensity at 7 days after the injury. To determine which cell types express testican-1 mRNA, we performed in situ hybridization histochemistry combined with immunohistochemistry of several cell markers. Testican-1 mRNA signals were detected in the proximal reactive astrocytes, whereas the distribution pattern of testican-1 mRNA positive cells was different from those of mature oligodendrocytes and activated microglia. In addition, signals for testican-1 mRNA overlapped with those of FGF-2 mRNA, showing that these molecules are coexpressed in reactive astrocytes. These results suggest a possibility that testican-1 plays a permissive role for regenerating axons in reactive astrocytes after injury. |
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