美洲凌霄花抗凝血功能研究

目的:研究美洲凌霄花的抗凝血作用,初步探讨其抗凝血机制.方法:采用断尾法和毛细管法分别测定小鼠尾出血时间(BT)、毛细管凝血时间(CT),并测定小鼠肺栓塞时间(PET),通过试剂盒测定小鼠体外凝血酶时间(TT)、凝血酶原时间(PT)、活化部分凝血激酶时间(APTT)、血浆复钙时间(PRT).结果:美洲凌霄花醇提物剂量依赖性延长小鼠CT、BT并剂量依赖性缩短小鼠肺栓塞时间(PET)；其不同萃取层中水层能显著延长小鼠CT、BT(P＜0.05),并能延长TT、PT、PRT(P＜0.05);粗多糖能够延长小鼠CT、BT,并能延长APTT、PT、PRT(P＜0.05).结论:美洲凌霄花具有抗凝血作用,其活性强度为多糖＞水层＞醇提物.

Research on Anti-coagulation Effect of the Flower of Campsis Radicans(L)Seem

Objective: To investigate the anti-coagulation activities effect of flower of Campsis radicans(L) Seem.Methods: Bleeding time(BT) and coagulation time(CT) was determined by cutting-tail method.And the pulmonary embolism time(PET) was also tested.Activated partial thromboplastin time(APTT),thrombin time(TT),prothrombin time(PT) and plasma recalcification time(PRT) were evaluated by test tube method in mice.Results: The bleeding time(BT) and coagulation time(CT) were prolonged and the pulmonary embolism time(PET) was cut short,which was dose dependent;In the five different extraction,the water extract can not only prolong the BT and CT(P<0.05),but also the PT,TT and PRT(P<0.05);polysaccharide extract prolonged the BT and CT,as well as the APPT,PT and PRT(P<0.05).Conclusion: The flower of Campsis radicans(L.)Seem has significant effect on anti-coagulation.The polysaccharide has stronger effect than the water extraction and acoparia-alcohol extract.And the the water extraction has stronger effect than acoparia-alcohol extract.