| 一氧化氮和氧自由基对培养大鼠皮层神经细胞的损伤 |
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| 引用本文： | 李晓梅,姚路禹,李天威,杨翰仪,窦春艳. 一氧化氮和氧自由基对培养大鼠皮层神经细胞的损伤[J]. 吉林大学学报(医学版), 1999, 25(1): 21-22 |
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| 作者姓名： | 李晓梅 姚路禹 李天威 杨翰仪 窦春艳 |
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| 作者单位： | 1. 基础医学院生物化学教研室,长春,130021
2. 第三临床学院 |
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| 摘 要： | 目的：探讨一氧化氮（ＮＯ）和氧自由基（Ｏ－２）对培养大鼠皮层神经细胞的损伤作用及两种损伤因素间的关系。方法：应用原代大鼠皮层神经细胞培养方法，研究外源性损伤因素的作用。结果：①以硝普钠（ＳＮＰ）为ＮＯ供体的损伤组较对照组的乳酸脱氢酶（ＬＤＨ）释放量显著增高，而细胞存活率显著降低，加入ＳＯＤ后ＳＮＰ的损伤作用减轻。②Ｏ－２损伤组的结果与ＮＯ损伤组结果相似，同时应用Ｏ－２和ＮＯ组对神经细胞的损伤作用显著高于单独应用Ｏ－２组或ＮＯ组。结论：ＮＯ和Ｏ－２都能造成培养的大鼠皮层神经细胞损伤，并且二者之间存在协同作用
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| 关 键 词： | 一氧化氮 氧自由基 神经细胞培养 |
| 修稿时间： | 1997-12-01 |
Damage to cultured rat cortical cells due to nitric oxide and superoxide |
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| Li Xiaomei,Yao Luyu,Li Tianwei,Yang Hanyi. Damage to cultured rat cortical cells due to nitric oxide and superoxide[J]. Journal of Jilin University: Med Ed, 1999, 25(1): 21-22 |
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| Authors: | Li Xiaomei Yao Luyu Li Tianwei Yang Hanyi |
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| Abstract: | Objective:To study the damage to cultured rat cortical cells due to nitric oxide (NO) and superoxide (O - 2),and to explore the relationship between NO and O - 2.Methods:Using primary cortical cell cultures to study damaging factors.Results:①Compared with control group,the Lactate Dehydrogenase (LDH) release caused by SNP (the provider of NO) increased significantly ( P <0 01),and the survival rate decreased significantly ( P <0 01).②The results of O - 2 damage to neuron were similar to those of SNP.More serious damage was caused by using NO and O - 2 together than by using NO or O - 2 separately ( P <0 01).Conclusion:Both NO and O - 2 can cause damage to cultured rat cortrical cells,and there is cooperation between them. |
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| Keywords: | nitric oxide superoxide cortical cell cultures |
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