魔芋葡甘聚糖/透明质酸钠复合材料构建组织工程髓核支架的实验研究

目的：构建一种新型的组织工程髓核支架材料，为应用组织工程技术修复退变椎间盘的研究奠定实验基础。方法以魔芋葡甘聚糖（konjac glucomannan，KGM）、透明质酸钠（Sodium hyaluronate，SH）为原料，应用真空冷冻干燥、化学交联等技术构建一种新型的组织工程髓核支架，同时对其理化性能及生物学、力学性能进行初步分析。结果支架材料大体及光学显微镜下观察呈三维立体多孔结构；扫描电子显微镜（ scanning electronic microscopy， SEM）观察显示支架材料为三维不均匀多孔结构；噻唑蓝比色法（MTT）分析结果显示支架材料生物相容性良好，无细胞毒性；体外生物降解性能检测结果显示支架材料具有良好的生物降解性能，12周时总降解率为87.6%；孔径为（572.5±51.8）μm，孔隙率为（82.1±7.5）%，吸水率为（1293.5±73.8）%；力学测试结果表明，支架材料具有一定的抗压强度。结论本研究制备的组织工程髓核支架材料具有理想的三维结构和理化性能，符合理想支架材料的结构要求，为进一步行体内外生物学性能研究提供了实验基础和依据。

A Empirical Study on Tissue-engineered Nucleus Pulposus Scaffold Constructed by KGM/SH Composite Material

Objective To construct a new material of tissue-engineered nucleus pulposus scaffold in order to provide the foundation for repairing degeneration intervertebral disk by tissue engineering. Methods A new tissue-engi-neered nucleus pulposus scaffold was constructed with konjac glucomannan(KGM)and sodium hyaluronate(SH)using techniques such as vacuum freeze-drying and chemical crosslinking,and the preliminary analysis for physico-chemical, biological and mechanical properties was performed. Results The bionic characteristics of scaffold showed three-dimen-sional porous structure by optical microscope observation,and three-dimensional asymmetrical porous structure by scan-ning electronic microscopy(SEM). The result of thiazolyl blue( MTT)colorimetry showed that the scaffolds had good biocompatibility without cytotoxicity. The detection of biodegradation capability in vitro showed that the scaffolds had good biodegradation capability,and the total biodegradation rate was 87. 6% in 12th week. The aperture,interval porosity and absorption rates were(572. 5 ± 51. 8)μm,(82. 1 ± 7. 5)% and(1293. 5 ± 73. 8)% . Biomechanical testing result showed that the scaffolds had certain compressive strength. Conclusion The tissue-engineered nucleus pulposus scaffold is an ideal scaffold material in three dimensional structure and physico-chemical function,and therefore it may provide basis for further studying biological capability in vitro.