胎盘多肽注射液对大鼠放射性肠炎防护作用的研究

目的：探讨胎盘多肽注射液对大鼠放射性肠炎的防护作用及机制。方法选择Wistar大鼠36只,随机分为4组,每组9只,A组为空白对照组,B组为生理盐水组、C组为地塞米松组(1.425 mg/kg)、D组为胎盘多肽组(0.08 ml/kg)。首先对各组大鼠进行药物预防灌胃,每日1次,每次灌6 ml。灌胃7 d后B、C、D组行下腹部一次性X-射线照射(剂量20 Gy),后继续灌胃7 d。应用血清超氧化物歧化酶( superoxide dismutase, SOD)、谷胱甘肽过氧化物酶( glutathione peroxidase, GSH-Px)和丙二醛( malondialdehyde, MDA)评估氧化应激水平,应用血管细胞黏附分子-1(vascular cell adhesion molecule-1, VCAM-1)、细胞间黏附分子-1(intercellular cell adhesion molecule-1, ICAM-1)、E选择素(E-selectin)和基质金属蛋白酶-9(matrix metallo protein-9, MMP-9)水平评估血管内皮细胞功能,并对各组大鼠进行整体状况和肠道评估。结果与A组相比,照射后B、C和D组大鼠均出现体重下降,且B和C组较D组下降更明显(P<0．05)。 B、C和D组大鼠照射后出现不同程度的肠道症状和肠道病理学改变,且B和C组大鼠改变较D组更明显。与A组相比,照射后B和C组SOD和GSH-Px水平明显下降( P<0．05), MDA、ICAM-1、VCAM-1、E-seletin和MMP-9水平明显升高(P<0．05)；D组大鼠SOD和GSH-Px水平高于B和C组,MDA、ICAM-1、VCAM-1、E-seletin和MMP-9水平低于B组和C组(P<0．05)。结论胎盘多肽注射液对放射性肠炎具有明确的防护作用,其可能是通过有效减少放射性肠炎过程中的氧化应激水平和改善血管功能产生作用。

A Study on Protective Effect of Placenta Polypeptide Injection in Treatment of Rats with Radiation Enterocolitis

Objective To investigate protective effect of Placenta Polypeptide Injection in treatment of rats with radiation enterocolitis and its mechanism. Methods A total of 36 wistar rats with weight between 250-300g were select-ed and randomly divided into 4 groups. Group A was blank control (n=9);group B was treated with 0. 9% physiologi-cal saline (n=9);group C was treated with Dexamethasone (1. 425mg/kg, n=9);group D was treated with Placenta Polypeptide (0. 08ml/Kg, n=9). All rats were given corresponding chemoprophylaxis by gavage 6 ml every time per day. X-radiation exposure of lower abdomen ( DT 20 Gy) for one time was performed after 7 d of gavage, and then ga-vaged was continued for another 7 d in group B, C and D. Oxidative stress levels was evaluated using superoxide dis-mutase ( SOD) , glutathione peroxidase ( GSH-Px) and malondialdehyde ( MDA) , and vascular endothelial cell function was evaluated using vascular cell adhesion molecule-1 (VCAM-1), intercellular cell adhesion molecule-1 (ICAM-1), E-selectin and matrix metallo protein-9 (MMP-9) level. General conditions and intestinal tract were also evaluated for all groups. Results Compared with those in group A, values of body weight in group B, C and D were decreased after ra-diotherapy, and the decreased values in group B and C were more obvious than that in group D (P<0. 05). After radio-therapy, rats in group B, C and D had different changes in symptoms and pathology of intestinal tract, and the changes in group B and C were more obvious compared with that in group D. Compared with those in group A, after radiotherapy SOD and GSH-Px levels in group B and C were significantly decreased (P<0. 05), while levels of MDA, ICAM-1, VCAM-1, E-selectin and MMP-9 were significantly increased (P<0. 05). In group D, SOD and GSH-Px levels were higher, while levels of MDA, ICAM-1, VCAM-1, E-selectin and MMP-9 were lower than those in group B and C (P<0. 05) . Conclusion Placenta Polypeptides injection has protective effect in treatment of radiation enterocolitis, and its mechanism may be that it can effectively reduce oxidative stress and improve vascular function.