胰高糖素样肽-1受体激动剂对甲状腺髓样癌细胞生长和增殖的影响

目的：探讨胰高糖素样肽-1(glucagon like peptide-1, GLP-1)受体在甲状腺髓样癌(medullary thyroid cancer, MTC)细胞系TT的表达情况及其对该细胞生长和增殖的影响。方法蛋白免疫印迹( Western blot)法检测TT细胞系GLP-1受体表达情况,采用甲基噻唑基四唑( MTT)评估两种GLP-1受体激动剂对细胞增殖及降钙素分泌的作用,Western blot法测定细胞信号通路相关蛋白的表达。结果 TT 细胞中可检测到 GLP-1受体的表达。1、10、100 nmol/L利拉鲁肽或艾塞那肽处理TT细胞后各时点的吸光度值与0 nmol/L比较差异无统计学意义,且相同浓度的两种药物在同一时间点对细胞增殖的作用比较差异无统计学意义(P>0．05)。不同浓度利拉鲁肽或艾塞那肽作用于TT细胞24 h后降钙素浓度比较差异均无统计学意义(P>0．05)。1、10、100 nmol/L利拉鲁肽和艾塞那肽作用于细胞24 h后Akt和Erk1/2的磷酸化水平与0 nmol/L比较无显著变化。结论人MTC细胞系TT中有GLP-1受体的表达。GLP-1受体激动剂对TT细胞的增殖、降钙素分泌及细胞信号通路相关蛋白的表达无明显影响。

Effects of Glucagon Like Peptide-1 Receptor on Cell Growth and Proliferation of Patients with Medullary Thy-roid Carcinoma

Objective To investigate expression of glucagon like peptide-1 (GLP-1) receptor in cell line TT and its effects on cell growth and proliferation of patients with medullary thyroid cancer (MTC). Methods Expression of GLP-1 receptor in cell line TT was detected using Western blot method. Effects of two kinds of GLP-1 receptor agonist on cell proliferation and calcitonin secretion were evaluated by methyl thiazolyl tetrazolium ( MTT) assay, and associated protein expressions of cell signal pathways were detected using Western blot method. Results GLP-1 receptor expression was detected in cell line TT. No significant differences of absorbance values at all time points were found in cell line TT after 1, 10 and 100 nmol/L Liraglutide or Exenatide treatment compared with those by 0 nmol/L Liraglutide or Exenatide treatment, and there was no significant difference between same concentration of Liraglutide or Exenatide groups at the same time (P>0. 05). No significant differences of calcitonin concentration were found after cell line TT was treated by different concentrations Liraglutide or Exenatide for 24 h ( P>0. 05 ) . No significant changes of Akt and Erk1/2 phos-phorylation levels were found after 1, 10 and 100 nmol/L Liraglutide or Exenatide treatment compared with those by 0 nmol/L Liraglutide or Exenatide treatment. Conclusion GLP-1 receptor can be expressed in human MTC cell line TT, but GLP-1 receptor agonist has no obvious effect on cell proliferation, calcitonin secretion and associated protein expres-sions of cell signal pathways.