胸苷激酶-1在直肠癌患者血清中的表达及其与临床病理特征的关系

目的探讨细胞质胸苷激酶-1(TK-1)在直肠癌患者血清中表达,分析其表达水平与临床病理特征之间的关系。方法回顾性分析了2008年12月—2012年12月在该院行手术治疗的60例直肠癌患者的临床资料,选择30例健康查体者为正常对照组,采用化学发光结合斑点杂交技术检测血清TK-1水平。结果直肠癌组、正常对照组血清TK-1水平分别为(2.96±0.99)pmol·L-1、(0.92±0.31)pmol·L-1,直肠癌组血清TK-1水平显示高于正常对照组,相比较差异有显著性(t=15.294,P0.05)。肿瘤低分化组血清TK-1水平显著高于肿瘤高中分组(t=8.412,P0.05),肿瘤≥6 cm组血清TK-1水平显著高于肿瘤6 cm组(t=9.865,P0.05),TNM分期Ⅲ～Ⅳ期组血清TK-1水平显著高于Ⅰ～Ⅱ期组(t=11.356,P0.05),发生淋巴结转移组血清TK-1水平显著高于淋巴结未转移组(t=9.842,P0.05)。结论 TK-1在直肠癌患者血清中呈高表达,血清TK-1的表达对直肠癌的诊断、肿瘤的侵袭和转移评估有一定临床意义。

Expression of serum thymidine kinase-1 and the relationship with clinicopathological features in patient with rectal cancer

Objective To investigate the expression levels of serum thymidine kinase-1（TK-l） and the relationship with clinicopathological features in patient with rectal cancer.Methods The clinical data of 60 patients with rectal cancer who underwent surgical treatment were retrospectively analyzed from December 2008 to December 2012,30 cases of healthy subjects considered as normal control group.The serum TK-1 levels were detected by chemiluminescence combined with dot blot hybridization.Results The serum TK-1 levels of rectal cancer group and normal control group were respectively（2.96 ± 0.99） pmol.L-1 and（0.92 ± 0.31） pmol.L-1,the serum TK-1 level of rectal cancer group was significantly higher than that of the normal control group（t = 15.294,P 0.05）.The serum TK-1 level of tumor poorly differentiated group was significantly higher than that of the tumor high and medium differentiated group（t = 8.412,P 0.05）.The serum TK-1 level of tumor ≥ 6 cm group was significantly higher than that of the tumor 6 cm group（t = 9.865,P 0.05）.The serum TK-1 level of stage Ⅲ ～ Ⅳ group was significantly higher than that of the stage Ⅰ ～ Ⅱ group（t = 11.356,P 0.05）.The serum TK-1 level of lymph node metastasis group was significantly higher than that of the without lymph node metastasis group（t = 9.842,P 0.05）.Conclusion The serum TK-1 level of patients with rectal cancer has high expression.The TK-1 expression level has clinical significance for diagnosing the rectal cancer and evaluating the tumor invasion and metastasis.