心肌缺血大鼠血浆8-异前列腺素F_(2α)水平及N-乙酰半胱氨酸干预作用(英文)

目的　8 异前列腺素F2α(8 iso PGF2α)是一种敏感、特异性反映缺血 再灌注后氧自由基增加的生 化指标。抗氧化剂N 乙酰半胱氨酸(NAC)具有清除氧自由基的作用。本研究旨在分析实验性心肌缺血大鼠血浆 与心肌8 iso PGF2α相关性以及NAC的治疗效果,探讨血浆8 iso PGF2α反映心肌氧自由基损伤程度的可能性和 NAC的干预效果。方法　45只雄性成年Wistar大鼠随机分为3组(每组15只):对照组、缺血组和NAC组。缺血 组和NAC组腹腔注射垂体后叶素(20U/kg)制成大鼠急性心肌缺血模型,以心电图上ST段的抬高作为心肌缺血 的指标。对照组仅腹腔注射生理盐水。NAC组缺血前2周开始用NAC(每日0.1g/kg)灌胃,共3周。应用ELISA 方法测定各组大鼠血浆及心肌组织8 iso PGF2α含量。结果　缺血组大鼠的血浆和心肌组织含量分别为(187.1± 45.8)pg/mL和(259.3±47.5)pg/g,明显高于正常对照组(60.4±13.7)pg/mL和(88.6±16.9)pg/g (P<0.01);NAC组的血浆和心肌组织8 iso PGF2α含量为(88.2±16.4)pg/mL和(109.4±24.7)pg/g明显低于 缺血组(P<0.01)。血浆与心肌8 iso PGF2α水平相关(r=0.856,P<0.01)。与正常组比较,缺血组的心电图 ST段明显抬高(心肌缺血45min时抬高最为明显,达0.34±0.05mV)(P<0.05);NAC组大鼠心

Significance of serum 8-iso-prostaglandin F2α level in acute myocardial ischemia and the therapeutic effect of N-acetylcysteine in rats

Objective 8-iso-8-iso-prostaglandin F 2α (8-iso-PGF 2α) is an index that can sensitively and specifically reflect peroxidation caused by increased free radicals after reperfusion. N-acetlycysteine (NAC) acts as an effective scavenger for free radical cleaning in the body. This study aimed to analyze the correlation of 8-iso-prostaglandin F 2α level between serum and myocardial tissue, and to investigate the therapeutic effect of NAC and the significance of serum 8-iso-PGF 2α level in rats with acute myocardial ischemia. Methods Forty-five male manhood Wistar rats were divided into three groups (15 rats in each group): Ischemia, NAC, and Control groups. Rats in the NAC group were given gastric lavage with NAC (0.1g/kg per day) for three weeks. Two weeks later the Ischemia and NAC groups were subjected to acute myocardial ischemia induced by intraperitoneal injection of pituitrin (20 U/kg), and the elevation of the ST segment in the ECG was used as the index of myocardial ischemia. The Control rats were injected intraperitoneally with normal saline. The 8-iso-PGF 2α contents in serum and myocardial tissue were determined by ELISA. Results Compared with the Control group, the 8-iso-PGF 2α contents in serum and myocardial tissue of the Ischemia group were significantly higher (60.4±13.7 pg/mL vs 187.4±45.8 pg/mL and 88.6±16.9 pg/mL vs 259.3±47.5 pg/g, both P< 0.01) In the NAC group, 8-iso-PGF 2α concentrations of serum and myocardial tissue were 88.2±16.4 pg/mL and 109.4±24.7 pg/g respectively, lower than those in the Ischemia group (P < 0.01). The 8-iso-PGF 2α levels of serum and myocardial tissue were positively correlated (r=0.865, P<0.01). In comparison with the controls, ST segments of ECG in rats with myocardial ischemia elevated and the peak of ST segments occurred 45 minutes after myocardial ischemia (0.34±0.05 mV) (P < 0.01). Pre-treatment with NAC improved myocardial ischemia (the elevation of ST segment was only 0.18±0.05 mV). Conclusions The level of 8-iso-PGF 2α increased in both serum and myocardial tissues of rats with acute myocardial ischemia. The serum level of 8-iso-PGF 2α can be used to evaluate the severity of myocardial ischemia. NAC reduces the free radical damage in myocardial tissues, and therefore, it may improve the amelioration of the heart during myocardial ischemia.