磁性纳米控释紫杉醇对食管癌Eca109细胞株生长的影响

目的:观察不同浓度磁性纳米控释紫杉醇对食管癌细胞Eca109的影响及与不同化疗药物的对比.方法:取对数生长期的食管癌Eca109细胞作细胞增殖抑制实验,实验组分别给予不同浓度的磁性纳米控释紫杉醇和5-氟尿嘧啶、泰素,同时设立二甲基亚砜(DMSO)和RPMI1640液对照,测定24,48,72h三个时间段的吸光度值,计算抑制率.经不同浓度的磁性纳米控释紫杉醇作用72h后,用电镜观察细胞超微结构,同时用流式细胞仪测定细胞周期和细胞凋亡.结果:MTT实验显示磁性纳米控释紫杉醇可抑制食管癌细胞增殖,与5-氟尿嘧啶,泰素相比,具有缓释性(P<0.01);电镜可发现药物作用组细胞核固缩、解聚以及凋亡小体;流式细胞仪检测显示G1峰前有明显的凋亡峰;细胞周期分析提示磁性纳米控释紫杉醇可将Eca109细胞阻滞于G2-M期,且与浓度相关.结论:磁性纳米控释紫杉醇对人食管癌细胞Eca109的生长有明显的抑制作用,使细胞分裂阻滞于G2-M期,并诱导细胞凋亡,且具有缓释效果.

Effects of magnetic nano-controlled release paclitaxel on the growth of esophageal carcinoma cell line Eca109

AIM: To observe the effects of magnetic nano- controlled paclitaxel at different concentrations on the growth of esophageal carcinoma cell line Eca109. METHODS: Eca109 cells in logarithmic growth phase were treated by magnetic nano-controlled release paclitaxel at different concentrations, fluorouracil (5-FU) and Taisu, while dimethyl- surfoxide (DMSO) and RPMI 1640 were used in the control cells. MTT assay was used to examine the inhibitory rate through the absor- bency of 24, 48, and 72 h. After Eca109 cells were disposed by magnetic nano-controlled release paclitaxel at different concentrations for 72 h, the cellular ultrastructure was observed under electron microscope and cell cycle and apoptosis were analyzed by flow cytometry. RESULTS: Magnetic nano-controlled releasepaclitaxel inhibited the proliferation of Eca109 cell in vitro, and the effect had characteristic of slower release in comparison with that of 5-FU or Taisu (78.1% vs 60.0%, 63.8%, P < 0.01). Chromation condensation, nucleic fragmenta- tion and apoptic body formation were found in drug-treated cells by electron microscopy. Flow cytometry analysis showed an obvious apop- totic peak before G1 peak and magnetic nano- controlled release paclitaxel blocked Eca109 cells in G2-M phase, which was associated with the concentrations. CONCLUSION: Magnetic nano-controlled re- lease paclitaxel can induce apoptosis and block cell cycle of esophageal cells.