结肠癌中P15,P16与MMP-9的表达及相关性

目的:研究P15,P16和MMP-9在结肠癌及正常组织中的表达,并探讨其与临床病理学特征的关系.方法:采用免疫组化法检测50例结肠癌组织、癌旁3cm组织及切缘正常组织中P15、P16和MMP-9表达的差异.结果:肿瘤、癌旁3cm与切缘正常组织中,P15的阳性表达率分别为84%,94%和100%,P16的阳性表达率分别为76%、90%和100%,MMP-9的阳性表达率分别为82%、44%和0,均有显著性差异(P<0.05).P15,P16和MMP-9的表达与结肠癌的分化程度、Dukes分期和淋巴结转移均有显著相关.结肠癌组织中MMP-9与P15、P16呈负相关性(r=-0.834,P<0.05;r=-0.752,P<0.05).结论:P15,P16表达的下调和MMP-9表达上调将促进结肠癌浸润和转移,二者在癌组织中表达的高低是结肠癌细胞重要的恶性生物学特征,有助于对结肠癌转移及患者预后的判断.

Expression of P15, P16 and matrix metalloproteinase-9 in colorectal cancer

AIM: To investigate the expression of P15/P16 and matrix metalloproteinase-9 (MMP-9) as well as their relationships with the clinicopathological characteristics in colorectal cancer. METHODS: Immunohistochemistry was used to detect the expression of P15, P16 and MMP-9 in 50 specimens of colorectal cancer tissues, tissues 3 cm away from cancer, and normal tissues from resection margin. RESULTS: The positive rates of P15 (84% vs 100%, P < 0.05), P16 (76% vs 100%, P < 0.05) and MMP-9 expression (82% vs 0, P < 0.05) were 84%, 76% and 82.00% in colorectal can- cer tissues, 94%, 90% and 44% in the tissues 3 cm away from cancer, and 100%, 100% and 0, respectively, and there were significant differ- ences among the three kinds of tissues (P < 0.05). The expression of P15/P16 and MMP-9 were markedly associated with the histological types, clinical stages and lymphatic metastases. More- over, MMP-9 expression was negatively corre- lated with P15 and P16 expression (r = -0.834, P< 0.05; r = -0.752, P < 0.05). CONCLUSION: Down-regulation of P15/P16 expression and up-regulation of MMP-9 play important roles in the invasion and metastasis of colorectal cancer, which can help to make judge- ment for metastasis and prognosis.