99m Tc 标记单克隆抗体Au14-1对荷宫颈癌小鼠的放射免疫显像研究

目的和方法：应用99mTc直接法标记抗小鼠宫颈癌（U14）单克隆抗体Au14-1，对荷瘤Km小鼠进行放射性分布和放射免疫显像的实验观察，探讨用99mTc－An14-1作宫颈癌导向诊断和导向治疗的可能性。结果：（1）99mTc－Au14-1注射后12h肿瘤灶已有放射性聚集，24h肿瘤组织的％ID／g值达8．79，呈明显特异性浓聚；（2）除肾脏外，各脏器的T／NT值在2．02~6．71之间，SPECT图像12h已见肿瘤显影，24h肿瘤影像更为清晰。结论：99TcTc－An14-1的体内分布与显像结果相一致，表明An14-1在体内具有良好的宫颈癌导向定位作用。

Radioimmunoimaging of 99m Tc labeled anti-mouse uterine cervical cancer monoclonal antibody Au14-1 in tumor-bearing mice

AIM and METHODS: A study of radioimmunoimaging was carried out on Kcnming mice - uterine cervical cancer (U14) using 99mTc labeled monoclonal Au14-1 with a modified Schwartaz method. RESULTS: The bio - distribution showed that radioactivity accumulated in tumor tissue at 12h after 99mTc - Au14- 1 injection in tail vein. The uptake by tumor was 4. 12 % ID/g at 2h and 8. 79 % ID/g at 24h respectively. The tumor/non - tumor (T/NT) radiocativity ratios for organs except kidneys were ranged from 2.02 to 6.71 at 24h post - injection. The image of tumor showed at 12h and clearer at 24h after injection. CONCLUSION: The quality of tumor image was relevant to the T/NT radioactivity ratios. It was demonstrated that 99mTc- Au14-1 has a good capability of localization for tumor.