Rab1A对NSCLC细胞迁移和JAK1磷酸化的影响

目的:观察Rab1A(Ras-related protein Rab-1A)对非小细胞肺癌(non-small cell lung cancer,NSCLC)细胞系迁移的影响及其分子机制.方法:应用siRNA干扰Rab1A基因表达;应用RTCA(real-time cell analysis)法分析干扰Rab1A基因表达对NSCLC细胞迁移速率的影响;应用免疫印迹观察干扰Rab1A基因表达对JAK1(Janus Kinase 1)磷酸化水平的影响.结果:与阴性对照组相比,Rab1A siRNA转染组NSCLC细胞系的Rab1A蛋白表达水平显著下调(P＜0.01);干扰Rab1A基因表达抑制NSCLC细胞迁移(P＜0.01);干扰Rab1A基因表达可下调NSCLC细胞迁移相关蛋白JAK1磷酸化水平(P＜0.01).结论:Rab1A通过调节JAK1磷酸化水平影响NSCLC细胞迁移.

Effect of Rab1A on the migraton of non-small cell lung cancer cell lines

Objective:To investigate the effects of Rab1A (Ras-related protein Rab-1A) on the migraton of non-small cell lung cancer (NSCLC) cell lines and its molecular mechanism.Methods:The siRNA interference was used to knockdown Rab1A.The effects of knocking Rab1A siRNA on the migraton of NSCLC cell lines were investigated by RTCA (real-time cell analysis).Western blot was used to analyze the effects of Rab1A on the phosphorylation level of JAK1 (P-JAK1).Results:Expression level of Rab1A protein was significantly down-regulated in Rab1A siRNA groups compared to negative control group (P ＜ 0.01).Rab1 A siRNA knockdown inhibited the migraton of NSCLC cell lines in Rab1 A siRNA groups compared to negative control group (P ＜ 0.01).Phosphorylation level of JAK1 (P-JAK1) was significantly decreased by Rab1A knockdown in Rab1A siRNA groups compared to negative control group (P ＜ 0.01).Conclusion:Rab1A promotes the migraton of NSCLC cell lines through regulating phosphorylation level of JAK1 (P-JAK1).