IRF-2对胰腺癌细胞生物学特性及化疗敏感性的影响

目的 探讨干扰素调控因子2(interferon regulatory factor 2,IRF-2)在胰腺癌细胞中的生物学特性及其对吉西他滨化疗敏感性的影响.方法 Western blot检测IRF-2基因在胰腺癌细胞株PANC-1及MIAPaCa-2中的表达水平,采用MTT检测吉西他滨对PANC-1及MIAPaCa-2的半数有效浓度(IC50),选择较为耐药的PANC-1细胞进行IRF-2基因干扰表达,并采用MTT检测吉西他滨对PANC-1及干扰IRF-2表达的PANC-1 si1#的半数有效浓度(IC50).结果 PANC-1及MIAPaCa-2中细胞中均存在IRF-2基因的表达,且PANC-1细胞中表达水平比MIAPaCa-2高.吉西他滨对PANC-1细胞的IC50显著高于MIAPaCa-2细胞,差异有统计学意义(P＜0.05);干扰IRF-2表达的PANC-1 si1#细胞其IC50值显著低于PANC-1对照细胞,差异有统计学意义(P＜0.05).结论 IRF-2基因可作为影响胰腺癌对吉西他滨化疗敏感性的基因之一,干扰IRF-2基因能够有效地提升胰腺癌细胞对吉西他滨化疗的敏感性.

Effect of IRF-2 on the Cellular Biological Characteristics and Chemosensitivity of Pancreatic Cancer

Objective To study the effect of IRF-2 on cellular biological characteristics and chemosensitivity of pancreatic cancer cells.Methods The expression level of IRF-2 in PANC-1 and MIAPaCa-2 cell lines were detected with Western blot,and the IC50 of PANC-1 and MIAPaCa-2 cell lines were detected with MTT.The IRF-2 gene were interfed in PANC-1 cell lines(named with PANC-1 si 1#),and the IC50 of PANC-1 and PANC-1 si 1# cell lines were also detected by MTT.Results PANC-1 and MIAPaCa-2 cells exist in IRF-2 gene expression,PANC-1 cells than MIAPaCa-2 high,gemcitabine PANC-1 cells median effective dose was higher than MIAPaCa-2,and the difference was statistically significant(P ＜ 0.05),the IC50 values of interference IRF-2-expressing cells was lower than PANC-1 control cells.Conclusion 1RF-2 gene is one of the gene which could influence the sensitivity of pancreatic cancer to gemcitabine chemotherapy,and IRF-2 specific interference technology could effectively enhance chemosensitivity pancreatic cancer cells to gemcitabine.