Survivin与p-mTOR在人肠癌组织中的表达及其相关性分析

目的:探讨人肠癌组织中Survivin与p-mTOR蛋白表达情况,并分析两者相关性.方法:首先应用免疫组织化学法检测86例肠癌组织中Survivin与p-mTOR蛋白的表达;然后利用Western blot技术检测4对冰冻肠癌组织及其癌旁组织中Survivin与p-mTOR蛋白的表达情况.结果:在免疫组化实验中,肠癌组织中Survivin蛋白的表达与组织学分级、淋巴结转移以及Dukes分期呈正相关(P＜0.05),与年龄、性别以及肿瘤大小无关(P＞0.05);而p-mTOR蛋白的表达与淋巴结转移和组织学分级也呈正相关(P＜0.01),与年龄、性别、肿瘤大小以及Dukes分期无关(P＞0.05).不仅如此,Survivin与p-mTOR在组织中的表达水平呈密切正相关.在Western blot实验中,肠癌组织Survivin与p-mTOR蛋白表达水平均明显高于癌旁组织,进一步说明Survivin与p-mTOR在肠癌组织中的表达水平上升且呈正相关.结论:肠癌组织中Survivin与p-mTOR蛋白的阳性表达与多项临床病理参数相关,并且两者表达也密切相关,提示其在肠癌进展中起到了重要作用,为探索肠癌诊疗新靶点提供理论依据.

Expression of Survivin and p-mTOR in colorectal cancer and the correlation of these two proteins

Objective:To investigate the expression of Survivin and p-mTOR proteins in colorectal cancer and analyze the correlation of these two proteins.Methods:Immunohistochemical technique was used to examine the expression of Survivin and p-mTOR proteins in 86 cases of colorectal cancer tissues.Relationship between the expression of Survivin and p-mTOR proteins and clinicopathological parameters were analyzed.Results:Elevated Survivin expression in colorectal cancer tissues was correlated with histological grade,lymph node metastasis and Dukes stage (P ＜ 0.05).High p-mTOR expression in colorectal cancer tissues was also correlated with lymph node metastasis and histological grade (P ＜ 0.01).Besides,high Survivin tended to indicate high p-mTOR expression from our correlation analysis.Conclusion:Survivin and p-mTOR were related to several important clinicopathological factors.Thus,they may serve as promising targets for therapy of colorectal cancer.