基质金属蛋白酶-9及血管内皮生长因子在卵巢癌组织中的表达及临床意义

目的:观察卵巢癌患者基质金属蛋白酶-9(matrix metalloproteinase-9,MMP-9)和血管内皮生长因子(vascular endothelial cell growth factor,VEGF)的表达水平,分析卵巢癌MMP-9和VEGF表达的临床意义.方法:将60份卵巢癌组织作为恶性组,60例卵巢良性肿瘤组织作为良性组,将获取的60份健康卵巢组织作为健康组,应用免疫组化法检测各组卵巢组织中MMP-9、VEGF阳性表达情况,应用RT-PCR检测不同卵巢组织中MMP-9 mRNA、VEGF mRNA相对表达量,应用Western blot法检测MMP-9和VEGF的蛋白水平,同时对比不同临床病理特征的卵巢癌组织中MMP-9、VEGF表达水平.结果:恶性组MMP-9阳性率(53.33%)、强阳性率(25.00%)和VEGF阳性率(55.00%)、强阳性率(26.67%)显著高于其他组(P<0.05),良性组MMP-9阳性率(23.33%)、VEGF阳性率(26.67%)显著高于健康组(P<0.05);恶性组MMP-9 mRNA、MMP-9蛋白、VEGF mRNA、VEGF蛋白显著高于其他组,其次为良性组,三组间MMP-9和VEGF表达水平差异显著(P<0.05);卵巢癌组织不同临床分期、不同淋巴结转移者的MMP-9、VEGF表达水平具有显著差异(P<0.05),且MMP-9表达和VEGF表达具有显著正相关性(P<0.05).结论:MMP-9、VEGF在卵巢癌中均为高表达,且二者表达强度与卵巢癌临床分期、淋巴结转移情况密切相关.

The expression and clinical significance of matrix metalloproteinase-9 and vascular en-dothelial growth factor in ovarian cancer tissue

Objective:To observe the expression of matrix metalloproteinase-9 (MMP-9)and vascular endo-thelial growth factor (VEGF)of oarian cancer patients,to analyze the clinical significance of MMP-9 and VEGF ex-pressions in ovarian cancer.Methods:60 ovarian cancer tissues were selected as malignant group,60 cases of benign ovarian tumor were selected as benign group and 60 healthy ovarian tissues were obtained as health group.Immunohis-tochemical method was applied to detect the MMP-9 and VEGF positive expressions in ovarian tissues.RT-PCR was applied to detect the relative expressions of MMP-9mRNA and VEGF mRNA in different ovarian tissues,the Western blot was applied to detect MMP-9 and VEGF protein levels.the MMP-9 and VEGF expression were com-pared in ovarian tissues with different clinical pathological characteristics.Results:In malignant group,MMP-9 positive rate (53.33%),strong positive rate (25.00%)and VEGF positive rate (55.00%),strong positive rate (26.67%) were significantly higher than other groups (P< 0.05).In benign group,MMP-9 positive rate (23.33%),VEGF positive rate (26.67%)were significantly higher than healthy group (P< 0.05).In malignant group,MMP-9 mR-NA,MMP-9 protein and VEGF mRNA,VEGF protein were significantly higher than other groups,followed by benign group.The differences expression of MMP-9 and VEGF were significant in the three groups (P< 0.05).There were significant differences in the expression of MMP-9 and VEGF in ovarian tissue with different clinical and lymph node metastasis (P< 0.05),and the expression of MMP-9 and VEGF had significant positive correlation (P< 0.05).Conclusion:MMP-9 and VEGF were highly expressed in ovarian cancer,and the expression intensity was closely re-lated to the clinical stages and lymph node metastasis of ovarian cancer.