A549细胞经2、4 Gy X线照射后miR-505表达变化的实验研究

目的:初步探讨2、4 Gy X射线照射后人肺腺癌A549细胞miR-505体内、外表达的变化及意义.方法:2、4 Gy X射线分别照射体外培养的A549细胞,以实时定量PCR(RT-qPCR)法检测A549细胞中miR-505表达水平.分别将0、2与4 Gy X射线照射后的A549细胞经尾静脉注射裸鼠制备裸鼠肺转移动物模型,检测裸鼠肺组织及血清中miR-505的表达水平.统计分析采用SPSS 19.0软件.结果:2 Gy X射线照射A549细胞后1、2、12、及24 h,miR-505表达均显著升高(升高倍数分别为:3.09、1.82、2.19及1.24倍,P<0.01);4 Gy X射线照射A549细胞后1、2、12 h,miR-505表达亦均显著升高(升高倍数分别为:2.99、2.06、1.86倍,P<0.01).0、2、4 Gy X线照射组裸鼠肺组织中均可检测到miR-505表达显著升高(升高倍数分别为:9.28、16.55及6.84倍,P<0.05);miR-505在血清中表达水平0 Gy和2 Gy组分别为5.33和3.78,与对照组相比显著升高(P<0.05).结论:2、4 Gy X射线照射可增加A549细胞miR-505的体内、外表达水平,可能与增强A549细胞体内、外侵袭转移能力相关.

An exprimental study of the expression of miRNA-505 after A549 cells were irradiated by 2 and 4 Gy X-ray

Objective:To investigate the expression of miR-505 in 2 and 4 Gy X-ray irradiated A549 cells in vitro and in vivo.Methods:Real-time quantitative PCR (RT-qPCR) assay was employed to detect the expression miR-505 in 2 and 4 Gy X-ray irradiated A549 cells in vitro and in vivo.Statistical analysis was performed using SPSS 19.0 software.Results:Compared with the control group,the expression of miR-505 were up-regulated significantly in X-ray irradiated A549 cells 1,2,12 and 24 hours post irraditaion,respectively (fold change was 3.09,1.82,2.19 and 1.24 with 2 Gy irradiation,P<0.01) and 1,2 and 12 hours post irraditaion respectively (fold change was 2.99,2.06 and 1.86 with 4 Gy irradiation,P<0.01).Furthermore,the expression of miR-505 was up-regulated in the lung and serum of nude mice with injection of 0,2 and 4 Gy X-ray irradiated A549 cells,compared with control group (fold change was 9.28,16.55 and 6.84 with 2 Gy irradiation,respectively,P<0.05).The expression of miR-505 were up-regulated in the serum of nude mice with injection of 0 and 2 Gy X-ray irradiated A549 cells,compared with control group (fold change was 5.33 and 3.78 with 2 Gy irradiation,respectively,P<0.05).Conclusion:2 and 4 Gy X-ray could up-regulated the expression of miR-505 of A549 cells,which might be correlated with the enhanced metastasis of A549 cells induced by X-ray in vitro and in vivo.