MCM7、Rb、c-MYC在肝细胞癌中的表达及其在基因通路中的作用

目的 探讨MCM7、Rb、c-MYC等基因在肝细胞癌中的表达及其在基因通路中的意义.方法 选取2013年1月至2015年8月邯郸市中心医院手术切除的60例原发性肝细胞癌组织进行免疫组化染色,分析MCM7、Rb、c-MYC等基因在肝细胞癌中的表达情况.结果 MCM7蛋白仅在肝细胞癌组织(73.3%)中表达,显著高于正常肝组织和肝硬化组织(P<0.05);Rb蛋白在肝硬化组织(53.3%)和肝细胞癌组织(58.3%)中表达,两者阳性率之间无统计学差异(P>0.05);肝细胞癌组织(70.0%)中c-MYC蛋白阳性率显著高于肝硬化组织(35.0%)(P<0.05).MCM7蛋白的表达与肿瘤直径、AFP、组织分化程度和临床分期有关(P<0.05),而与病灶数、是否转移及HBsAg是否阳性无关(P>0.05);Rb蛋白的表达仅与肿瘤组织分化程度有关(P<0.05);c-MYC蛋白表达与肿瘤组织分化程度和是否转移有关(P<0.05).MCM7蛋白和Rb阳性率呈显著负相关(γ=-0.712,P=0.038;γ=-0.664,P=0.018).结论 肝细胞癌的发生是多基因多通路相互作用的结果,多种调控蛋白的异常共同导致肿瘤的发生.

Expression of MCM7,Rb,c-MYC Protein in Hepatocellular Carcinoma Tissues and Its Effect on Gene Passageway

Objective To investigate the expression of MCM7,Rb,c-MYC protein in hepatocellular carcinoma tissues and its effect on gene passageway .Methods Tumor tissues of 60 cases of hepatocellular carcinoma were performed immunohisto-chemistry stain,the expression of MCM7,Rb and c-MYC genes in hepatocellular carcinoma were analyzed .Results MCM7 pro-tein expressed only in HCC tissue(73.3%),which was significantly higher than normal liver tissues and liver cirrhosis tissues (P<0.05).Rb protein expressed in liver cirrhosis (53.3%) and HCC tissues(58.3%),the positive rates between the 2 tissues had no statistical difference(P>0.05).Positive rate of c-MYC protein in HCC tissue(70.0%) was significantly higher than that in liver cirrhosis tissues(35.0%)(P<0.05).The expression of MCM7 was related to tumor size,AFP,differentiation degree and clinical stage(P<0.05),and not related to tumor foci,metastasis and HBsAg(P>0.05).The expression of Rb was related to differentiation degree only(P<0.05).The expression of c-MYC was related to differentiation degree and metastasis (P<0.05). The positive rate of MCM7 was negatively correlated to that of Rb protein (γ=-0.712,P=0.038;γ=-0.664,P=0.018). Conclusion The occurrence of hepatocellular carcinoma is the consequence of polygene and multichannel .Anomaly of multiple protein co-induce the occurrence of hepatocellular carcinoma .