| CCK-8法与LDH法检测CIK细胞毒活性的比较 |
| |
| 引用本文： | 肖晓君,赵盼,钟克力,戴勇,邹畅. CCK-8法与LDH法检测CIK细胞毒活性的比较[J]. 现代肿瘤医学, 2017, 0(16): 2530-2533. DOI: 10.3969/j.issn.1672-4992.2017.16.002 |
| |
| 作者姓名： | 肖晓君 赵盼 钟克力 戴勇 邹畅 |
| |
| 作者单位： | 1. 深圳市人民医院超声科,广东 深圳,518020;2. 深圳市人民医院临床医学研究中心,广东 深圳,518020;3. 深圳市人民医院胃肠科,广东 深圳,518020 |
| |
| 基金项目： | 国家自然科学基金(81402370)，广东省自然科学基金(2015A030313829)，广东省科技项目产学研合作项目(2015B090904007)，广东省科技厅粤港创新圈项目(2016A050503009) |
| |
| 摘 要： | 目的:探讨CCK-8法和LDH法检测CIK细胞活性的最佳条件.方法:经密度梯度法分离得到人外周血中单个核细胞,经细胞因子刺激培养14 天收获CIK细胞,采用CCK-8法与LDH法检测CIK细胞对前列腺癌细胞毒活性.结果:CCK-8法的最佳检测时间为加入CCK-8试剂后3~4 h,适宜细胞数量范围为0.32×103~2.0×104个/孔.结论:CCK-8法较LDH法稳定性好,准确性好,操作简便,是一种优于LDH法的检测免疫细胞毒活性的方法.
|
| 关 键 词： | 前列腺癌 CCK-8 LDH CIK |
Comparison of the cytotoxic activity of CIK cells by CCK-8 and LDH assay |
| |
| Xiao Xiaojun,Zhao Pan,Zhong Keli,Dai Yong,Zou Chang. Comparison of the cytotoxic activity of CIK cells by CCK-8 and LDH assay[J]. Journal of Modern Oncology, 2017, 0(16): 2530-2533. DOI: 10.3969/j.issn.1672-4992.2017.16.002 |
| |
| Authors: | Xiao Xiaojun Zhao Pan Zhong Keli Dai Yong Zou Chang |
| |
| Abstract: | Objective:To investigate the optimal conditions of CCK-8 and LDH assay for detecting CIK cell cytotoxic activity.Methods:Peripheral blood mononuclear cells(PBMCs)were isolated by Ficoll-Paque gradient centrifugation and CIK cells were amplified by cytokine.After culturing 14 days,the cytotoxicity of CIK cells to prostate cancer cells was detected by CCK-8 and LDH assay.Results:The best detection time of CCK-8 method was 3~4 h after adding CCK-8 reagent,and the suitable cell number range was 0.32×103~2.0×104 cells/hole.Conclusion:CCK-8 is a better method than LDH in stability,accuracy and operation to detect the cytotoxic activity of immune cells. |
| |
| Keywords: | prostate cancer CCK-8 LDH CIK |
| 本文献已被 万方数据 等数据库收录！ |
|
