PD-L1及TGF-β在非小细胞肺癌中的表达及其临床意义

目的 探讨在非小细胞肺癌(NSCLC)中PD-L1及TGF-β表达水平,及其与NSCLC临床病理参数和术后无病生存期(DFS)的关系.方法 收集81例NSCLC标本,采用免疫组化方法检测术后肿瘤组织中PD-L1、PD-1、Foxp3+和TGF-β蛋白的表达水平.结果 81例患者中PD-L1阳性表达为46例(56.8%),阴性表达35例(43.2%).TGF-β阳性36例(44.4%),阴性45例(55.6%).PD-1阳性33例(40.7%),阴性48例(59.3%);Foxp3+阳性44例(54.3%),阴性37例(45.7%).NSCLC中PD-L1蛋白表达与各临床病理参数无明显相关性.单因素分析发现T分期、TGF-β表达、PD-L1表达影响.SCLC术后DFS,具有统计学差异(P<0.05),多因素分析发现T分期、TGF-β表达、PD-L1表达是影响NSCLC术后DFS的独立因素(P<0.05).PD-L1阳性表达组术后DFS为(21.000±1.429)个月,阴性表达组为(14.500±1.615)个月,两组有统计学差异(χ2=6.930,P=0.008).TGF-β阳性组PFS为(14.500±0.813)个月,阴性组为(21.000±1.639)个月,两组有统计学差异(χ2=8.71,P=0.003).结论 非小细胞肺癌中PD-L1及TGF-β蛋白是预测NSCLC术后DFS的重要指标,且PD-L1表达越高预示术后DFS越长,而TGF-β则表达越高,预示术后DFS越短.

Expression of PD-L1 and TGF-βin Non-small Cell Lung Cancer and Its Clinical Significance

Objective To investigate the expression of PD-L1 and TGF-β in non-small cell lung cancer ( NSCLC) ,and its relationship with clinical pathological parameters and disease free survival ( DFS) of NSCLC after operation. Methods 81 specimens of NSCLC were collected,Immunohistochemical method was used to detect the levels of PD-L1,PD-1,Foxp3 +and TGF-β protein in tumor tissues after operation. Results In 81 patients,the positive expression of PD-L1 was 46 cases(56. 8%), and the negative expression was 35 cases(43. 2%). TGF-βpositive in 36 cases (44. 4%),negative in 45 cases (55. 6%). PD-1 positive in 33 cases (40. 7%),negative in 48 cases (59. 3%),Foxp3+positive in 44 cases (54. 3%),negative in 37 cases (45. 7%). The expression of PD-L1 protein in NSCLC was not significantly correlated with clinicopathological parameters. Single factor analysis Indicate that the T staging,the expression of TGF-β,and the expression of PD-L1 influenced the postoperative DFS of NSCLC,and there was a statistical difference (P<0. 05),Multivariate analysis showed that the T stage,the expression of TGF-β and the expression of PD-L1 were independent factors influencing the postoperative DFS of NSCLC (P<0. 05). The postopera-tive DFS in the PD-L1 positive expression group was (21+1. 429) months,and the negative expression group was (14. 500+1. 615) months,and the difference between the 2 groups was statistically significant (χ2 =6. 930,P=0. 008). In the TGF-βposi-tive group,DFS was (14. 500+0. 813) months,and the negative group was (21+1. 639) months,and the difference between the 2 groups was statistically significant (χ2 =8. 71,P=0. 003). Conclusion PD-L1 and TGF-β protein in NSCLC are important indexes to predict the DFS of NSCLC,and the higher the expression of PD-L1,the longer the postoperative DFS,the higher the ex-pression of TGF-β,the shorter the postoperative DFS.