血清中CD44v5及c-met检测对食管鳞癌高危人群的筛检价值

目的:检测食管鳞癌患者CD44v5及c-met表达情况,并分析其在癌组织及癌旁组织的表达差异;探讨采用ELISA法检测两者在血清中含量用于食管鳞癌筛检的价值.方法:采用RT-PCR技术和Western-Blot法分别检测CD44v5及c-met基因在食管鳞癌组织及癌旁组织中的表达;并采用酶联免疫吸附法(ELISA法)测定CD44v5及c-met蛋白在100例食管鳞癌患者及60例健康体检者血清的含量.结果: CD44v5、c-met mRNA在食管鳞癌组织中IA值(0.737±0.116,0.748±0.122)高于相应癌旁食管组织(0.140±0.065,0.147±0.064),差异均具有统计学意义(P<0.01);CD44v5及c-met蛋白相对表达量在癌组织(1.099±0.230,1.054±0.227)中亦高于相应癌旁组织(0.265±0.105,0.245±0.097)(P<0.01);CD44v5蛋白在食管鳞癌患者血清中的含量(31.308±10.123) μg/L]高于健康体检者(19.364±1.680) μg/L],差异具有统计学意义(P<0.01),c-met蛋白在食管鳞癌患者血清中的含量(101.183±15.335) μg/L]亦高于健康体检者(56.165±8.979) μg/L](P<0.01).CD44v5与c-met在食管鳞癌患者血清中的含量具有相关性(r=0.880,P<0.01).ELISA法检测CD44v5、c-met、二者并联及串联诊断食管鳞癌的ROC曲线下面积分别为0.865、1.000、0.800及0.950,灵敏度分别为91.0%、99.0%、100.0%、90.0%,特异度分别为60.0%、100.0%、60.0%、100.0%.结论:CD44v5与c-met的高表达与食管鳞癌的发生有关,二者具有相关性;ELISA法检测血清中c-met蛋白含量,并联合CD44v5蛋白检测,或许有助于在食管鳞癌高发地区对高危人群进行大规模筛检.

The detection of CD44v5 and c-met in serum for screening of high-risk population of esophageal squamous cell carcinoma

Objective:To detect the expression of CD44v5 and c-met in esophageal squamous cell carcinoma (ESCC),and analyze the difference between the carcinoma tissues and adjacent tissues.Explore value of screening for ESCC by detecting the contents of CD44v5 and c-met using ELISA method.Methods:To detect the expression of CD44v5 and c-met in ESCC tissues and its corresponding adjacent non-carcinomatous tissues by RT-PCR and Western-Blot,and use enzyme-linked immunosorbent assay (ELISA) to detect the proteins in serum of 100 ESCC patients and 60 healthy subjects.Results:CD44v5 and c-met mRNA IA value in ESCC tissues (0.737±0.116,0.748±0.122) were both higher than that of esophageal tissues adjacent to carcinoma (0.140±0.065,0.147±0.064)(P<0.01).The content of CD44v5 and c-met proteins in cancerous tissues (1.099 ±0.230,1.054±0.227) were higher than tissues adjacent to carcinoma (0.265±0.105,0.245±0.097)(P<0.01) respectively.And in serum,CD44v5 and c-met proteins in patients with ESCC (31.308±10.123) μg/L,(101.183±15.335) μg/L] were higher than healthy subjects (19.364±1.680) μg/L,(56.165±8.979) μg/L] respectively,and the differences were all statistically significant (P<0.01).There was some correlation between CD44v5 expression and c-met in patients with ESCC (r=0.880,P<0.01).The AUC of the ROC curves of diagnosis of ESCC using ELISA method to detect CD44v5,c-met,both in parallel and series were 0.865,1.000,0.800 and 0.950 respectively.The sensitivity of the diagnosis were 91.0%,99.0%,100.0% and 90.0% respectively,and the specificity were 60.0%,100.0%,60.0% and 100.0% respectively.Conclusion:The high expression of CD44v5 and c-met is associated with the occurrence of ESCC,and there's correlation between them.Using ELISA method to detect the contents of c-met in serum,and combining with CD44v5 protein detection,may contribute to mass screening of ESCC in high-risk groups in the areas of prone to ESCC.