三氧化二砷对人子宫内膜癌细胞裸鼠模型中Caspase-3表达的影响

目的 通过建立人子宫内膜癌细胞HEC-1-B裸鼠模型,检测不同浓度三氧化二砷(As2 O3)对移植瘤细胞Caspase-3表达的影响,并探讨As2 O3的作用机制.方法 建立人子宫内膜癌HEC-1-B细胞皮下移植瘤模型,按编号随机分为实验组即As2O3低(1.0 mg/kg)、中(2.5 mg/kg)、高(5.0 mg/kg)剂量组、空白对照生理盐水(NS)组及阳性对照顺铂组(5.0 mg/kg),连续腹腔给药8天后,通过流式细胞仪及实时荧光定量RT-PCR检测移植瘤内Caspase-3相对表达量.结果 与生理盐水组比较,低、中浓度As2O3及顺铂均可上调Caspase-3的表达(P<0.05),但高浓度组上调不明显(P>0.05).与顺铂组比较,As2O3低剂量组作用最明显,差异有统计学意义(P<0.05);但与中、高浓度组比较,差异无统计学意义.结论 低浓度As2 O3能上调人子宫内膜癌细胞内Capase-3的表达,且优于顺铂,可能是三氧化二砷诱导细胞凋亡的作用机制之一.

EFFECT of Arsenic Trioxide on Caspase-3 in Nude Mice Models of Human Endometrial Carcinoma Cell Lines(Hec-1B)

Objective To study the effect of arsenic trioxide ( As2 O3 ) on expression of Caspase-3 in nude mice models of human endometrial carcinoma cell lines (Hec-1B).Methods The nude mice models of endometrial carcinoma cell lines (Hec-1B)were randomized into 5 groups via number,then they were treated with NS,DDP,increasing concentrations of As2O3(1.0 mg/kg,2.5 mg/kg,5.0 mg/kg) by intraperitoneal injection.The Caspase-3 expression was detected by flow cytometry and real-time PCR.Results Compared with NS group,the As2O3(1 mg/kg、2.5 mg/kg)groups and DDP group increased significantly the ex-pression of Caspase-3(P<0.05).But As2O3(5 mg/kg)group hadn't distinguished effection.Compared with DDP group,the As2O3(1 mg/kg)group was statistically significant(P<0.05),but the As2O3(2.5 mg/kg、5 mg/kg)groups weren't.Conclusion (1 mg/kg)As2O3 can increase the expression of Caspase-3,and was superior to DDP,the action may be one of mechanisms of ar-senic trioxide inducing apoptosis.