趋化因子受体CcR5在结肠癌中的表达及临床意义

目的 探究趋化因子受体CcR5在结肠癌中的表达及临床意义.方法 收集经结肠癌根治术切除且经病理学检查证实为结肠癌组织标本94例,选取50例距离肿瘤边缘>5 cm,且经病理学检查为正常的癌旁组织作为对照,采用实时定量PCR和免疫组化的方法,分别检测结肠癌组织和正常癌旁组织中CcR5的mRNA表达水平及阳性表达率,分析其与结肠癌临床病理学特征的关系.结果 结肠癌组织中CcR5 mRNA水平和阳性表达率明显高于正常癌旁组织的,差异具有统计学意义(P<0.05).CcR5 mRNA表达水平和阳性表达率与肿瘤大小、淋巴结转移、肝转移、TNM分期均有关,肿瘤大小>5 cm、出现淋巴结转移和肝转移、Ⅲ~Ⅳ期结肠癌患者CcR5的mRNA表达水平和阳性表达率均相应高于肿瘤大小≤5 cm、未发生转移、Ⅰ~Ⅱ期患者(P<0.05).LMVD与肿瘤大小、淋巴结转移、肝转移、TNM分期和分化程度有关.相关性分析结果显示,CcR5的mRNA表达水平与LMVD呈明显正相关(γ=0.9176,P=0.0099).结论 趋化因子受体CcR5的高表达与结肠癌发生、转移均有关,检测CcR5对于结肠癌的诊断和预测转移情况具有指导意义.

Expression of Chemokine Receptor CcR5 in Colorectal Cancer and Its Clinical Significance

Objective To explore the expression of chemokine receptor CcR 5 in colorectal cancer and clinical signifi-cance .Methods 94 cases of colorectal cancer specimens treated with colorectal cancer resection surgery confirmed by pathology examination were selected .Meanwhile ,50 specimens of cancer adjacent normal tissues from the edge of the tumor >5 cm were the control group.The mRNA level of CcR5 and positive case of CcR5 in colorectal cancer tissues and normal adjacent tissues were detected using real-time quantitative PCR and immunohistochemistry method .The relationship of CcR 5 mRNA level and positive case of CcR5 with clinicopathological features in colorectal cancer were analyzed .Results The mRNA level of CcR5 and positive case of CcR5 in colorectal cancer tissues were significantly higher than that in normal adjacent tissues ,there showed statistically significant difference(P<0.05).mRNA expression level of CcR5 and positive case of CcR5 were both related with tumor size, lymph node metastasis,liver metastasis,TNM stage,which showed that CcR5 mRNA level and positive case of CcR5 in patients with tumor size>5 cm,lymph node metastasis and liver metastasis ,Ⅲ~Ⅳperiod were markedly higher than that in patients with tumor size ≤5 cm,no transfer,Ⅰ~Ⅱperiod(P<0.05).LMVD related to tumor size,lymph node metastasis,liver metastasis, TNM stage and differentiation degree .Correlation analysis showed that the expression level of mRNA was positively correlated with LMVD(γ=0.9176,P=0.0099).Conclusion The high expression of chemokine receptors CcR 5 is related to the occurrence and metastasis of colorectal cancer .Detection of CcR5 has instructive value for diagnosis and metastasis prediction of colorectal cancer.