| EFFECTS AND MECHANISMS OF ENDOSTATIN ON THE GROWTH OF OVARIAN CANCER SKOV3 CELLS IN VITRO AND IN VIVO |
| |
| 引用本文： | 刘梅梅 隋丽华 李佩玲 程丽. EFFECTS AND MECHANISMS OF ENDOSTATIN ON THE GROWTH OF OVARIAN CANCER SKOV3 CELLS IN VITRO AND IN VIVO[J]. 中国癌症研究, 2006, 18(2): 132-137,141 |
| |
| 作者姓名： | 刘梅梅 隋丽华 李佩玲 程丽 |
| |
| 作者单位： | [1]Department of Gynecology ＆ Obstetrics, The Second Affiliated Hospital of Harbin Medical University,Harbin 150086 [2]Department of Gynecology, The Third Affiliated Hospital of Harbin Medical University,Harbin 150040 |
| |
| 摘 要： | Objective： To evaluate the inhibitory effect of Endostatin on ovarian cancer cell line SKOV3 and to investigate the possible mechanism of the inhibition. Methods： Using MTT, transmission electron microscope （TEM） and immunocytochemistry, the effects of Endostatin on the proliferation of SKOV3 cells were studied. Nude mice were subcutaneously implanted with SKOV3 cells. The cell apoptosis of implanted tumor was detected by TUNEL and TEM. The expressions of bcl-2 and bax in implanted tumor tissues were measured by RT-PCR and immunohistochemistry. Results： Endostatin significantly inhibited the proliferation of SKOV3 cells in vitro （P〈0.01） and induced cell apoptosis, whereas the expressions of bcl-2 and bax were not changed obviously in SKOV3 cell treated with Endostatin. The mean tumor weight of Endostatin treated group was markedly lower than that of PBS control group （P〈0.05）. The expression of bcl-2 was down-regulated in Endostatin treated group, but bax was not influenced. Conclusions： The results demonstrated that Endostatin might have anti-tumor effect on ovarian carcinoma. One of the important mechanisms of Endostatin effect of anti-angiogenic and anti-tumor activities might involve regulating the bcl-2/bax expression and inducing apoptosis.
|
| 关 键 词： | 卵巢癌 细胞凋亡 免疫细胞化学 病理机制 |
| 文章编号： | 1000-9604（2006）02-0132-06 |
| 收稿时间： | 2005-12-14 |
| 修稿时间： | 2006-03-29 |
EFFECTS AND MECHANISMS OF ENDOSTATIN ON THE GROWTH OF OVARIAN CANCER SKOV3 CELLS IN VITRO AND IN VIVO |
| |
| LIU Mei-mei,SUI Li-hua,LI Pei-ling,CHENG Li. EFFECTS AND MECHANISMS OF ENDOSTATIN ON THE GROWTH OF OVARIAN CANCER SKOV3 CELLS IN VITRO AND IN VIVO[J]. Chinese Journal of Cancer Research, 2006, 18(2): 132-137,141 |
| |
| Authors: | LIU Mei-mei SUI Li-hua LI Pei-ling CHENG Li |
| |
| Abstract: | Objective: To evaluate the inhibitory effect of Endostatin on ovarian cancer cell line SKOV3 and to investigate the possible mechanism of the inhibition. Methods: Using MTT, transmission electron microscope (TEM) and immunocytochemistry, the effects of Endostatin on the proliferation of SKOV3 cells were studied. Nude mice were subcutaneously implanted with SKOV3 cells. The cell apoptosis of implanted tumor was detected by TUNEL and TEM. The expressions of bcl-2 and bax in implanted tumor tissues were measured by RT-PCR and immunohistochemistry. Results: Endostatin significantly inhibited the proliferation of SKOV3 cells in vitro (P<0.01) and induced cell apoptosis, whereas the expressions of bcl-2 and bax were not changed obviously in SKOV3 cell treated with Endostatin. The mean tumor weight of Endostatin treated group was markedly lower than that of PBS control group (P<0.05). The expression of bcl-2 was down-regulated in Endostatin treated group, but bax was not influenced. Conclusions: The results demonstrated that Endostatin might have anti-tumor effect on ovarian carcinoma. One of the important mechanisms of Endostatin effect of anti-angiogenic and anti-tumor activities might involve regulating the bcl-2/bax expression and inducing apoptosis. |
| |
| Keywords: | Ovarian cancer Implanted tumors Endostatin SKOV3 cell Cell apoptosis Nude mice |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
