砷对小鼠卵母细胞成熟和体外受精的影响

目的　探讨三氧化二砷（Ａｓ２Ｏ３） 对小鼠生殖作用的影响。方法　采用小鼠卵母细胞体外培养、体外受精的方法研究Ａｓ２Ｏ３ 对卵母细胞成熟和体外受精的影响。结果　Ａｓ２Ｏ３ 对小鼠卵母细胞生发泡破裂、超排卵的卵母细胞数目没有影响， 但可以抑制卵母细胞第一极体的释放， 降低体外受精率和卵母细胞的存活率。结论　Ａｓ２Ｏ３ 可以破坏卵母细胞的成熟、降低卵母细胞的受精能力， 对生殖细胞和生育能力有一定的毒性作用。

The effect of Arsenic on oocyte maturation and in vitro fertilization in mice oocyte

Objective To examine whether the arsenic affects the reproduction of mouse.Method By using the methods of mouse oocyte culture and in vitro fertilization the effect of arsenic on oocyte maturation and in vitro fertilization(IVF)was studied.Results Arsenic could inhibit the extruding of the first polar body,reduce the quality and the viability of oocyte and lower the rate of IVF,but only little impact on the germinal vesicle breakdown(GVBD)and the number of oocytes after induction of ovulation.Conclusion The results suggest that arsenic may obstruct oocyte maturation in mice that reduces the fertile ability of oocyte,therefore it is toxic to oocyte and fertility ability in mice.