| 亚硒酸钠与细胞的作用 |
| |
| 引用本文： | 温和瑞 陈荣三. 亚硒酸钠与细胞的作用[J]. 微量元素与健康研究, 1998, 15(2): 6-7,26 |
| |
| 作者姓名： | 温和瑞 陈荣三 |
| |
| 作者单位： | [1]江西赣南师范学院化学系，赣州341000 [2]南京大学配位化 |
| |
| 摘 要： | 通过测定巨噬细胞的存活率、脂质过氧化、细胞内游离Ｃａ２＋、Ｍｇ２＋的浓度和红细胞膜Ｃａ２＋－ＡＴＰ酶和Ｍｇ２＋－ＡＴＰ酶的活性，研究了亚硒酸钠与细胞的作用。结果表明高浓度的Ｎａ２ＳｅＯ３（≥１０－４ｍｏｌ·Ｌ－１）有明显的细胞毒性；Ｎａ２ＳｅＯ３对巨噬细胞的毒性作用引起细胞内游离Ｃａ２＋、Ｍｇ２＋浓度升高；但Ｍｇ２＋浓度升高比Ｃａ２＋慢；较高浓度的Ｎａ２ＳｅＯ３（≥１０－５ｍｏｌ·Ｌ－１）能抑制红细胞膜Ｃａ２＋－ＡＴＰ酶和Ｍｇ２＋－ＡＴＰ酶的活性，但对Ｍｇ２＋－ＡＴＰ酶活性的抑制作用要低于对Ｃａ２＋－ＡＴＰ酶活性的抑制作用。
|
| 关 键 词： | 亚硒酸钠 细胞毒性 脂质过氧化 细胞内游离Ca~(2＋)、Mg~(2＋) Ca~(2＋)-ATP酶 Mg~(2＋)-ATP酶 |
Effects of Sodium Selenite on Cell |
| |
| Abstract: | The effects of Na2SeO3 on macrophage were studied by means of the survival percentage of macrophage,the lipid peroxides,the cytoplasmic free Ca2+and Mg2+concentration,the activity of Ca2+-ATP ase and Mg2+-ATP ase of erythrocyte membrane.The results showed that the high concentration Na2SeO3(≥10-4mol·L-1)had the cytotoxicity.The poisonous effect of Na2SeO3 on macrophage led to the increase of cytoplasmic free Ca2+and Mg2+concentration,but the increase of cytoplasmic free Mg2 concentration was slower than that of cytoplasmic free Ca2+concentration.High concentration Na2SeO3 inhibited the Ca2+-ATPase and Mg2+-ATPase activity.But the inhibition to Mg2+-ATPase was Weaker than that to Ca2+-ATPase. |
| |
| Keywords: | Sodium Selenite Cytotoxicity Lipid Peroxides Cytoplasmic free Ca2+、Mg2+ Ca2+-ATPase Mg2+-ATPase |
| 本文献已被 CNKI 维普 等数据库收录！ |
