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Induction of anti-leukemic cytotoxic T lymphocytes by fusion of patient-derived dendritic cells with autologous myeloblasts |
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| Authors: | Gong Jianlin Koido Shigeo Kato Yoko Tanaka Yasuhiro Chen Dongshu Jonas Anna Galinsky Ilene DeAngelo Daniel Avigan David Kufe Donald Stone Richard |
| Affiliation: | a Dana–Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA b Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02115, USA |
| Abstract: | Presentation of AML antigens by dendritic cells (DC) could potentially induce a T cell-mediated anti-leukemic immune response. In the present study, we generated DC from adherent (AD-DC) and non-adherent (NAD-DC) myeloblasts obtained from bone marrows of AML patients. Both cell populations displayed morphological, phenotypic and functional properties of DC. The functions of NAD-DC were compared to AD-DC that had been fused with autologous AML blasts (FC/AML). The FC/AML induced greater T cell proliferation and CTL activity against autologous AML blasts (9/10 cases) as compared to NAD-DC. FC/AML may thus represent a promising strategy for DC-based immunotherapy of patients with AML. |
| Keywords: | Generation of dendritic cells Fusion of autologous DC and AML blasts Autologous T cell proliferation Anti-leukemia immunity |
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