CD4+Treg细胞在急性髓细胞白血病中的变化及其意义

目的 研究Treg细胞在急性髓细胞白血病(AML)中的表达及其意义,并探讨Treg细胞在AML治疗前与缓解后的区别和联系.方法 流式细胞仪检测AML初治组(23例)、AML完全缓解(CR)组(23例)及正常对照组(22例)CD4+CD25+T细胞和CD4+FOXP3+T细胞分别占CD4+T细胞的比例,采用直接免疫荧光法标记T细胞特征抗原.结论 CD4+ FOXP3+T细胞占CD4+T细胞的比例:AML初治组明显高于正常对照组(P<0.01)和完全缓解组(P<0.01),而完全缓解组与正常对照组差异无统计学意义(P>0.05);CIM+CD25+T细胞占CD4+T细胞的比例:经检验在各组中差异没有统计学意义(P>0.05).结论CD4+Treg细胞数量在初治AML中明显升高,治疗达完全缓解后恢复正常.

Alterations and significance of CD4+ Treg cell in acute myelocytic leukemia

Objective To explore the alterations and significance of CD4~+ regulatory T cells(Treg cells) in acute myelocytic leukemia (AML) and to approach the distinction and association of Treg cells between untreated and complete remission (CR) AML patients. Methods The percentages of CD4~+ CD25 ~+ T cells and CD4~+ FOXP3~+ T cells in CD4~ + T cells were examined by direct immunofluorescence straining technique and detected by flow cytometry in 46 AML patients including 23 untreated and 23 complete remission patients. 22 healthy persons were tested as normal control. Result The proportions of CD4~+ FOXP3~+ T cells were much higher in untreated AML patients than in normal control (P <0.01) and CR group (P <0.01). But there was no significant difference in the percentages of CD4~+ FOXP3~+ T cells between normal controls and CR group(P >0.05). There was no significant difference in the percentages of CD4~+ CD25~+ T cells between any groups (P > 0. 05). Conclusion The quantity of CD4~+ Treg cells is obviously increased in untreated AML patients and the abnormality can be recovered in complete remission patients.