氧合血红蛋白对脑微血管内皮细胞增殖和凋亡的影响

目的 探讨氧合血红蛋白（OxyHb）对体外培养的大鼠脑微血管内皮细胞增殖和凋亡的影响。方法 将原代培养的大鼠脑血管内皮细胞与不同浓度的氧合血红蛋白共孵育,以MTT法检测脑血管内皮细胞的存活率;将氧合血红蛋白与脑血管内皮细胞共培养,以流式细胞仪和Hochest33258荧光染色法检测细胞凋亡的情况。结果 氧合血红蛋白能显著抑制血管内皮细胞的增殖,与对照相比存在统计学意义,能显著诱导血管内皮细胞凋亡。结论 在一定浓度范围内的OxyHb能够明显抑制原代培养的脑血管内皮细胞增殖并引起凋亡,提示SAH后内皮细胞凋亡可能是发生CVS的原因之一。

Effect of oxyhaemoglobin on proliferation and apoptosis of brain vascular endothelial cells in vitro

Objective To investigate the effect of oxyhaemoglobin(OxyHb)on proliferation and apoptosis of cultured rat brain microvascular endothelial cells(RBMvECs).Methods Primary cultured RBMvECs were incubated with various concentrations of OxyHb for various times.Cell proliferation ability was assessed by MTT method,cell apoptosis was measured by flow cytometry and Hochest33258 fluorescent stain methods.Results OxyHb can significantly inhibit the proliferation and induced apoptosis of RBMvECs compared with control.The RBMvECs apoptotic rate were related to the concentration of OxyHb and cultured time.Conclusions OxyHb can induced primary cultured RBMvECs apoptosis at certain concentrations which suggests apoptosis of endothelial cells might be one of the causes of CVS after SAH.