高强度聚焦超声损伤离体恶性胶质瘤的研究

目的探讨高强度聚焦超声(h igh intensity focused u ltrasound,H IFU)体外治疗恶性胶质瘤的有效性、安全性及最佳声学剂量。方法根据不同的辐照剂量,将15例离体恶性胶质瘤标本分为10 s组、20 s组和30 s组,用频率9.7MHz,焦距4.5 mm,声强2 500 W/cm2的H IFU以不同剂量进行定点辐照,行病理组织学切片及电镜对比观察。结果10 s组胶质瘤组织辐照区无明显变化;20 s组辐照区凝固性坏死,边缘区无明显变化;30 s组辐照区及边缘区均凝固性坏死。结论在本试验设定参数情况下,20 s为最佳声学剂量,H IFU能够准确有效杀灭胶质瘤,而对未辐照的瘤组织无影响。

High intensity focused ultrasound ablation for human malignant gliomas in vitro

Objective To explore the efficiency,safety and suitable dosage of the non-invasive treatment of malignant gliomas with high intensity focused ultrasound(HIFU).Methods An extracorporeal HIFU with 9.7 MHz transducer(focal length: 4.5 mm,focal intensity: 2 500 W/cm~(2)) was used.Fifteen resected human malignant gliomas were randomly assigned to receive HIFU ablation for 10,20,30 s.After the treatment,the exposure coverage and marginal zone were collected and examined by HE staining and electron microscope.Results Exposure coverage and margin was safe in 10 s group.Typical coagulation necrosis was detected at exposure coverage with no detectable change at margin in 20 s group.Typical coagulation necrosis was seen at both exposure coverage and margin in 30 s group.Conclusion Under the parameters used in this study,20 s is a beneficial therapeutic dosage.HIFU is an accurate method for malignant gliomas ablation.