牵拉对α1受体激动剂苯肾上腺素诱发兔离体血管收缩反应的影响

目的研究在α1受体激动剂苯肾上腺素(Phe)诱发血管收缩反应实验中,如何确定动脉标本的最适前负荷.方法采用兔离体肾动脉、股动脉、隐动脉、肠系膜动脉、脾动脉和耳中央动脉环标本的等长张力纪录法.结果在1.0～5.0 g前负荷条件下, 随前负荷增加, 各血管环静息张力呈线性增加. KCl (120 mmol·L-1)引起各动脉的收缩随前负荷的增加而增强,但除耳动脉外, 在其他动脉标本上未观察到KCl最大收缩反应的最终坪值点.以Phe(0.01～100 μmol·L-1)为收缩剂时, 在肠系膜动脉, 脾动脉和隐动脉, Phe的EC50(EC50·Phe)值随前负荷增加(1.0～5.0 g)而明显改变; 但是, 各组标本在不同前负荷下的EC50·Phe值中存在一个最小值,其标准差亦很小.结论前负荷明显影响血管的EC50·Phe值测定.确定离体血管标本最适前负荷, 特别是研究受体反应时, 应以受体激动剂的EC50值及其变异程度最小作为关键指标.

Influence of stretch on α1 receptor agonist phenylephrine regulated vasoconstriction in rabbit regional arteries

AIM To investigate how to determine the optimal preloads in the study of α1 receptor agonist phenylephrine (Phe) regulated vasoconstriction in the rabbit isolated arteries. METHODS Vasoconstrictive responses to Phe were recorded in the rabbit renal, femoral, saphenous, mesenteric, splenic and ear arteries. RESULTS The resting tension in various arterial rings was increased with increasing preload from 1.0 g to 5.0 g in a linear manner. The vasoconstrictive responses to KCl (120 mmol·L-1) in the regional arteries were increased with increasing preload, however, the final plateau of the Emax of KCl was not observed in these regional arteries, with the exception of the ear artery. In the experiment with Phe (0.01-100 μmol·L-1) as a vasoconstrictive agent, the EC50 values of Phe (EC50·Phe) in the rabbit mesenteric, splenic and saphenous arteries were changed largely by changing preload, however there was an optimal point where the EC50·Phe value and its standard deviation were minimal among the five values corresponding to 1.0 g to 5.0 g preloads in each artery. CONCLUSION The EC50 values of Phe in the rabbit isolated arteries are influenced by preloads obviously. In the determination of optimal preload in isolated arterial preparations, particularly in the study of receptor regulated function, the minimal EC50 value and its standard deviation of corresponding-receptor agonist should be considered as a crucial factor.