高效液相色谱法测定丹参中丹酚酸B等3种有效成分

 目的测定丹参中丹酚酸B，丹参素和原儿茶醛等3种水溶性有效成分的含量。方法采用高效液相色谱法测定。以甲醇-5％冰醋酸为流动相进行梯度洗脱，以对羟基苯甲酸为内标，检测波长为281 nm，柱温30℃。结果 丹参素、原儿茶醛和丹酚酸B浓度分别在3.95～47.4 μg·mL^-1(r=0.999 6)，5.14～61.68μg·mL^-1(r=0.999 9)，16.8～117.6 μg·mL^-1(r=0.999 4)范围内呈良好线性关系，丹参素、原儿茶醛和丹酚酸B的平均回收率分别为101.43%(RSD=2.88%，n=6)，99.89%(RSD=3.04%，n=6)和103.43%(RSD=1.52%，n=6)。结论方法简便，分离效果好，能同时测定丹参中丹酚酸B、丹参素、原儿茶醛等3种水溶性有效成分的含量，结果准确可靠。

Determination of three effective components in Salvia miltiorrhiza by means of HPLC

OBJECTIVE To investigate the determination of three water soluble components in Salvia miltiorrhiza. METHODS The analysis was carried on a column of Hypersil C₁₈(250 mm×4.6 mm,5 μm) with a mobile phase of methanol-5% acetic acid (gradient elution from 10∶90 to 35∶65 in 20 minutes),using 4 hydroxy benzoic acid as the internal standard.The detection wavelength was at 281 nm. RESULTS The linearity was obtained over 3.95～47.4 μg·mL^-1(r=0.999 6)for Danshensu,5.14～61.68 μg·mL^-1(r=0.999 9)for protocatechuic aldrhyde and 16.8～117.6 μg·mL^-1(r=0.999 4) for salviamolic acid B,respectively.The average recoveries were 101.43%(RSD=2.88%，n=6) for Danshensu,99.89%(RSD=3.04%，n=6)for protocatechuic aldrhyde and 103.43% (RSD=1.52%，n=6) for salviamolic acid B,respectively. CONCLUSION This method appeared to be rapid,simple and accurate.