| 荷瘤大鼠60Co局部照射后骨髓蛋白质的差异表达 |
| |
| 引用本文： | 李萡,海春旭,秦绪军,何伟,梁欣,李文丽,张晓迪,赵康涛,陈宏莉,刘瑞. 荷瘤大鼠60Co局部照射后骨髓蛋白质的差异表达[J]. 医学争鸣, 2004, 25(14): 1332-1334 |
| |
| 作者姓名： | 李萡 海春旭 秦绪军 何伟 梁欣 李文丽 张晓迪 赵康涛 陈宏莉 刘瑞 |
| |
| 作者单位： | 第四军医大学预防医学系毒理学教研室,陕西,西安,710033;第四军医大学预防医学系毒理学教研室,陕西,西安,710033;第四军医大学预防医学系毒理学教研室,陕西,西安,710033;第四军医大学预防医学系毒理学教研室,陕西,西安,710033;第四军医大学预防医学系毒理学教研室,陕西,西安,710033;第四军医大学预防医学系毒理学教研室,陕西,西安,710033;第四军医大学预防医学系毒理学教研室,陕西,西安,710033;第四军医大学预防医学系毒理学教研室,陕西,西安,710033;第四军医大学预防医学系毒理学教研室,陕西,西安,710033;第四军医大学预防医学系毒理学教研室,陕西,西安,710033 |
| |
| 摘 要： | 目的:观察荷瘤大鼠受60Co局部照射后骨髓中蛋白质的差异表达.方法:制备荷瘤大鼠动物模型,60Co局部照射后分别提取荷瘤组和照射组大鼠骨髓蛋白质,运用双向聚丙烯酰胺凝胶电泳技术分析2组荷瘤大鼠骨髓蛋白质的差异表达.结果:荷瘤大鼠经60Co局部照射骨髓有24个蛋白点表达发生变化,其中出现2个新蛋白点,4个蛋白点表达量下降,1个蛋白点表达量升高,17个蛋白点在照射后消失.结论:60Co局部照射可诱导荷瘤大鼠骨髓蛋白质差异表达.
|
| 关 键 词： | 照射 大鼠 骨髓 电泳 凝胶 双向 蛋白质类 |
| 文章编号： | 1000-2790(2004)14-1332-03 |
| 修稿时间： | 2004-03-23 |
Differential expression of bone marrow proteins in tumor-bearing rats after 60Co local irradiation |
| |
| Abstract: | AIM: To study the differential expression of bone marrow proteins (BMPr) after 60 Co local irradiation in tumor bearing rats. METHODS: Entitative tumor pieces were embedded into rat hind leg muscle to prepare tumor bearing rat model. After 60 Co local irradiation, the bone marrows of rats in both tumor bearing and irradiated groups were removed to extract BMPr, respectively. Two dimensional polyacrylamide gel electrophoresis was used to analyze the differential expression of BMPr. RESULTS: Alterations were found in 24 proteins in tumor bearing rats after 60 Co local irradiation, of which 2 novel proteins, 4 down regulated proteins, 1 up regulated protein and 17 proteins that disappeared following irradiation. CONCLUSION: The differential expression of BMPr can be induced by 60 Co local irradiation in tumor bearing rats. |
| |
| Keywords: | irradiation rats bone marrow electrophoresis gel two dimensional proteins |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
