miR-579在肾癌中表达的临床意义及其对肾癌细胞增殖的影响

目的 探讨微小RNA-579(miR-579)在肾癌中表达的临床意义及其生物学功能.方法 选取2012年1月—2014年3月四川省内江市第一人民医院泌尿外科手术治疗肾癌患者92例,收集其癌组织及癌旁正常组织,检测miR-579表达水平;比较不同临床病理特征癌组织中miR-579水平;对不同miR-579水平的肾癌患者进...

Clinical significance and biological function of miR-579 in renal carcinoma.

Objective To explore clinical significance and biological function of microRNA-579(miR-579)in renal carcinoma.Methods The samples of cancer tissues and adjacent normal tissues in ninety-two patients with renal carcinoma who underwent operation in the Urology Department of the First People's Hospital of Neijiang City,Sichuan Province from January 2012 to March 2014 were collected.The expression of miR-579 in tissues were detected.The levels of miR-579 in cancer tissues of patients with different clinicopathological characteristics were compared.The survival rates of patients with different miR-579 levels were compared.Renal cancer A498 cells were randomly divided into three groups:control group,miR-NC group and miR-579 group.The expression of miR-579,the level of proliferation,and the expression of 3’-phosphoinositide-dependent protein kinase-1(PDK1)and murine double minute 2(MDM2)were compared.Results The expression level of miR-579 in cancer tissues was lower than that in adjacent tissues(t/P=16.010/0.000).,but related to tumor diameter(t/P=3.420/0.001),differentiation degree and clinical stage(F/P=10.514/0.000,7.308/0.000).The survival rate of the low expression group was lower than that of the high expression group(χ²/P=4.684/0.030).Compared with control group and miR-NC group,the expression level of miR-579 in miR-579 group increased(F/P=480.200/0.000),the OD value decreased at 48 and 72 hours after culture(F/P=12.870/0.000,13.040/0.000),and the expression levels of PDK1 and MDM2 decreased(F/P=67.500/0.000、18.780/0.000).Conclusion The low expression of miR-579 in renal carcinoma tissues and cells is related to tumor diameter,differentiation degree,clinical stage and low survival rate.MiR-579 mimics in vitro can inhibit cell proliferation,which may be related to the inhibition of PDK1 and MDM2 expression.