miR-124通过靶向调控AURKA抑制胶质瘤细胞的增殖

目的:探索miR-124及AURKA对胶质瘤细胞增殖能力的影响并探索其机制.方法:通过PCR测定胶质瘤组织及非肿瘤组织中miR-124、AURKA的表达水平;将携带miR-124的脂质体miR-124 mimic、空载体miR-124转染入胶质瘤细胞系LN-229和T98G中,吸光度观察miR-124对胶质瘤细胞系LN...

miR-124 inhibits the proliferation of glioma cells through targeted regulation of AURKA

Objective:To explore the effects of miR-124 and AURKA on the proliferation of glioma cells and explore its mechanism.Methods:The expression levels of miR-124 and AURKA in glioma tissues and non-tumor tissues were determined by PCR.Liposome miR-124 mimic carrying miR-124 and empty vector miR-124 were transfected into glioma cell line LN-229 and T98G,the effect of miR-124 on the proliferation of glioma cell lines LN-229 and T98G was observed by absorbance.The target gene of miR-124 was predicted by Targetscan software,and the binding site of miRNA-124 and AURKA was detected by Luciferase report.Real-time quantitative PCR method detects the changes of AURKA gene after miRNA-124 transfection.Results:Compared with non-glioma tissues,the expression of miR-124 was decreased in glioma tissues.MTT experiments showed that the proliferation ability of glioma cells in the high expression group of miR-124 was weakened.The proliferation ability of glioma cells in the miR-124 low expression group increased,the difference is statistically significant(P<0.05).Targetscan software suggested that AURKA may be the target gene of miR-124.Western blot and Luciferase experiments showed that compared with the negative control group,the expression of AURKA in the high expression group of miR-124 decreased,while the expression of AURKA in the low expression group of miR-124 increased.Conclusion:miR-124 is down-regulated in glioma cells and tissues,and may affect the proliferation and invasion of glioma cells by regulating AURKA.