微小RNA Let-7在子宫肌瘤组织中的表达情况及与复发相关危险因素的探讨

目的:探讨单发、多发子宫肌瘤、不同直径子宫肌瘤以及肌瘤与相应肌壁组织中miRNA Let-7的表达情况,探讨miRNA Let-7与子宫肌瘤复发危险因素的关系.方法:运用实时定量聚合酶链反应(real-time,PCR)方法分别检测单发肌瘤与多发子宫肌瘤、不同直径的肌瘤组织以及对应肌壁组织中miRNA Let-7的相对...

The expression of microRNA Let-7 in uterine fibroids and the risk factors related to recurrence

Objective:To investigate the expression levels of microRNA Let-7 in tissues of uterine myoma and surrounding normal myometriumin,all cases were classified into different groups according to the number or diameter of myomas,then analyze the relations between microRNA Let-7 and risk factors of myoma recurrence.Methods:The microRNA let-7 expression levels in uterine myoma and surrounding normal myometrium in tissues were detected by quantitative real-time PCR from groups of single or multiple uterine myoma,larger myomas(diameter≥5 cm) or smaller myomas(diameter<5 cm).Results:The expression levels of microRNA let-7 in myoma tissues were slightly weaker than that of surrounding normal myometriumin,but the difference was not significant(P>0.05).Then the expression levels of microRNA let-7 in groups of single myoma and smaller myoma(diameter<5 cm) were higher than of multiple myoma and larger myoma(diameter≥5 cm),and the difference was statistically significant(P<0.01).Conclusion:The number and diameter of myomas are independently risks of recurrence.MicroRNA Let-7 with higher expression in multiple myoma and larger myoma is expected to be an new marker for myoma recurrence.