牙源性囊肿上皮增殖活性的研究

目的：研究牙源性角化囊肿，含牙囊肿，根尖囊肿3种主要的牙源性囊肿衬里上皮的细胞增殖活性，方法：应用Ki-67单克隆抗体免疫组化LSAB法对30例牙源性囊肿进行免疫组化染色，结果通过计算机图像分析，计算单位面积衬里上皮内（mm2)阳性细胞数，进行统计学分析，结果：牙源性角化囊肿衬里上皮有较多的Ki-67阳性细胞，明显高于含牙囊肿和根尖囊肿；正常口腔粘膜未见Ki-67阳性表达，结论：Ki-67在不同的牙源性囊肿中表达的差异显示了它们具有不同的增殖和分化过程。

Cell Proliferation in Odontogenic Jaw Cyst Epithelium

OBJECTIVE: The aim of the study is to investigate the epithelial cell proliferation in the lining of odontogenic cysts with Ki-67 monoclonal antibody. METHODS: Ki-67 expression was studied in 30 odontogenic cysts (odontogenic keratocysts, n = 10; dentigerous cyst, n = 10; radicular cysts, n = 10), and normal oral epithelium (n = 10) by using a biotin streptavidin method on routinely processed paraffin sections after microwave treatment. Ki-67+ cells were counted manually and related to the area of epithelial lining as determined by computer image analyzer. The result was analyzed by using a computer image analyzer to calculate positive cells per square millimeter of the epithelial lining of odontogenic lesions. Data were analyzed by using student test. RESULTS: The epithelial lining of odontogenic keratocysts contained the highest number of Ki-67+ cells, most of which were located in the suprabasal layers. The mean value of Ki-67+ cells in odontogenic keratocysts linings (1812.29 +/- 606.47 cells/mm2) were significantly higher than that of dentigerous (944.43 +/- 58.98 cells/mm2) and radicular cysts (610 +/- 26.4 cells/mm2) (P < 0.01). Negative reactions were obtained in the normal oral epithelium. CONCLUSION: The results demonstrate that there are differences in Ki-67 expression between epithelial linings of the major odontogenic cysts, which were consistent with odontogenic keratocysts having a greater proliferative capability than dentigerous and radicular cysts. It also indicates differences in proliferation and differentiation processes within these lesions.