黄芪甲甙下调CaSR对异丙肾上腺素诱导的缺血缺氧心肌损伤的保护作用

目的 探讨黄芪甲甙介导钙敏感受体(CaSR)对缺血缺氧心肌的保护作用及机制。方法 将32只SD大鼠随机分为正常对照组、模型组(IS0)、黄芪甲甙低剂量组(ISO+AST IV 10 mg/kg)、黄芪甲甙高剂量组(ISO+AST IV 20 mg/kg)；HE染色光镜下观察不同组别心肌组织的病理学改变,检测不同组别血清肌酸激酶及肌酸激酶同工酶含量；Western blotting法检测CaSR蛋白的表达。结果 与模型组相比黄芪甲甙低、高剂量组能不同程度减轻心肌病理改变,降低血清肌酸激酶以及肌酸激酶同工酶含量,下调CaSR蛋白表达水平(P＜0.05)。结论 黄芪甲甙对异丙肾上腺素诱导大鼠缺血缺氧心肌有保护作用；其机制可能为下调CaSR蛋白表达减轻CaSR对心肌损伤的作用。

The peotective effect of astragaloside IV mediated calcium sensitive receptor on ischemia anoxic myocardial induced by isoproterenol

Objective To explore the protective effects and mechanism of Astragaloside IV mediated calcium sensitive receptor (CaSR) on ischemia anoxic myocardial.Methods 32 SD rats were randomly divided into normal control group,model group (IS0) glucoside,astragaloside IV low dose group (ISO + AST IV 10 mg/kg),astragaloside IV high dose group (ISO + AST IV 20 mg/kg).Observing myocardial tissue pathology change of different groups by HE staining,detecting CK and CK-MB content; detecting CaSR protein expression by Western Blotting method.Results Compared with model group astragaloside IV low dose group and high dose group can reduce the myocardial pathological varying degrees changes,reduce serum CK and CK-MB,lower CaSR protein expression levels (P< 0.05).Conclusion Astragaloside IV can protect ischemia anoxic myocardial injury induced by ISO; The mechanism may reduce the CaSR protein expression and effects on myocardial injury.