| 血清学业民DNA方法用于人类白细胞抗原—Ⅰ类分型的比较研究 |
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| 引用本文： | 谭建明 唐孝达. 血清学业民DNA方法用于人类白细胞抗原—Ⅰ类分型的比较研究[J]. 中华医学杂志, 2000, 80(3): 187-189 |
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| 作者姓名： | 谭建明 唐孝达 |
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| 作者单位： | 南京军区福州总医院肾移植中心(谭建明!350025福州),上海市第一人民医院肾移植中心(唐孝达,谢桐) |
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| 基金项目： | 中国博士后科学基金资助项目,国家卫生部科研基金资助项目!( 962 2 89) |
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| 摘 要： | 双盲比较血清学和聚合酶链反应－单链构象多态性分析方法用于汉族人群人类白细胞抗原１（ＨＬＡ－Ⅰ）类分型的结果。方法临床样本５２５份,采用微量淋巴细胞毒技术血清学方法和ＰＣＲ－ＳＳＰ技术ＤＮＡ方法行ＨＬＡ－Ａ、Ｂ抗原分型,比较其准确性、重复性和临床实用性。
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| 关 键 词： | HLA－A抗原 HLA－B抗原 DNA 白细胞抗原 分型 |
| 修稿时间： | 1999-03-26 |
Comparisonof HLA class |
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| TAN Jianming ,TANG Xiaoda,XIE Tong. Renal Transplant Center,Fuzhou General Hospital,Fuzhou ,China. Comparisonof HLA class[J]. Zhonghua yi xue za zhi, 2000, 80(3): 187-189 |
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| Authors: | TAN Jianming TANG Xiaoda XIE Tong. Renal Transplant Center Fuzhou General Hospital Fuzhou China |
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| Affiliation: | Renal Transplant Center, Fuzhou General Hospital, Fuzhou 350025, China. |
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| Abstract: | OBJECTIVE: To compare HLA class I typing by serology with PCR-SSP method in the Chinese population. METHODS: HLA-A and -B antigens were typed by serology with microlymphocytotoxicity and DNA typing with PCR-SSP in 525 clinical samples. Reliability, reproducibility and clinical practicability of both methods were compared according to the typing results. RESULTS: Serological typing for HLA class I was rapid and simple, costing 3 hours. The discrepancy rate between serology and PCR-SSP for HLA-A antigen was 9.0%, consisting of 21 antigens being incorrectly interpreted by serology and 26 of serological "blanks" turning out to be definable alleles by DNA typing. The discrepancy rate for HLA-B antigen was 12.2%, containing 39 antigens being incorrectly interpreted and 35 of serological "blanks" turning out to be definable alleles by PCR-SSP typing. DNA typing for HLA class I by PCR-SSP proved to be an accurate, reliable and well-reproducible technique within 5 hours. While it was difficult to suit clinical assay by a large scale screening because of complicated operation and high technical condition. CONCLUSION: DNA typing for HLA class I by PCR-SSP is suitable for clinical application in the Chinese population with a higher precision than serology. Screening test by serology is recommended. Ambiguous or blank antigens by serology should be retyped by DNA typing. |
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| Keywords: | Antigens HLA-A Antigens HLA-B Serology DNA |
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