| Rapamycin Modulates the Maturation of Rat Bone Marrow-derived Dendritic Cells |
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| 引用本文： | 丁英俊,程翔,唐婷婷,姚瑞,陈勇,谢江娇,余娴,廖玉华. Rapamycin Modulates the Maturation of Rat Bone Marrow-derived Dendritic Cells[J]. 华中科技大学学报(医学英德文版), 2008, 28(4): 391-395. DOI: 10.1007/s11596-008-0405-1 |
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| 作者姓名： | 丁英俊 程翔 唐婷婷 姚瑞 陈勇 谢江娇 余娴 廖玉华 |
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| 作者单位： | Institute of Cardiology，Union Hospital，Tongji Medical College，Huazhong University of Science and Technology |
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| 基金项目： | 国家重点基础研究发展规划(973计划)，国家自然科学基金 |
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| 摘 要： | The purpose of the study was to observe the effect of rapamycin （RAPA） on the differentiation and maturation of rat bone marrow-derived dendritic cells （BMDCs） in vitro. BMDCs from Wistar rats were cultured with granulocyte-macrophage colony-stimulating factor plus interleukin-4 in the presence or absence of RAPA （20 ng/mL）, and stimulated with lipopolysaccharide （LPS） for 24 h before cells and supernatants were collected. Surface phenotype of BMDCs was flow-cytometrically detected to determine the expression of maturation markers, MHC class Ⅱ and CD86. Supernatants were analyzed for the production of IL-12 and IFN-γ cytokines by using ELISA. BMDCs were co-cultured with T cells from Lewis rats and mixed lymphocyte reaction was assessed by MTT method. The morphology of BMDCs stimulated with LPS remained immature after RAPA pretreatment. RAPA significantly decreased the CD86 expression, impaired the IL-12 and IFN-γ production of BMDCs stimulated with LPS, and inhibited the proliferation of allogeneic T cells. In conclusion, RAPA can inhibit the maturation of BMDCs stimulated with LPS in terms of the morphology, surface phenotype, cytokine production, and ability of BMDCs to stimulate the proliferation of allogeneic T cells in vitro.
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| 关 键 词： | 树突细胞 骨髓 脂多糖 BMDCs |
Rapamycin modulates the maturation of rat bone marrow-derived dendritic cells |
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| Yingjun Ding,Xiang Cheng,Tingting Tang,Rui Yao,Yong Chen,Jiangjiao Xie,Xian Yu,Yuhua Liao. Rapamycin modulates the maturation of rat bone marrow-derived dendritic cells[J]. Journal of Huazhong University of Science and Technology. Medical sciences, 2008, 28(4): 391-395. DOI: 10.1007/s11596-008-0405-1 |
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| Authors: | Yingjun Ding Xiang Cheng Tingting Tang Rui Yao Yong Chen Jiangjiao Xie Xian Yu Yuhua Liao |
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| Affiliation: | Institute of Cardiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022,China |
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| Abstract: | The purpose of the study was to observe the effect of rapamycin (RAPA) on the differentiation and maturation of rat bone marrow-derived dendritic cells (BMDCs) in vitro. BMDCs from Wistar rats were cultured with granulocyte-macrophage colony-stimulating factor plus interleukin-4 in the presence or absence of RAPA (20 ng/mL), and stimulated with lipopolysaccharide (LPS) for 24 h before cells and supernatants were collected. Surface phenotype of BMDCs was flow-cytometrically detected to determine the expression of maturation markers, MHC class II and CD86. Supernatants were analyzed for the production of IL-12 and IFN-gamma cytokines by using ELISA. BMDCs were co-cultured with T cells from Lewis rats and mixed lymphocyte reaction was assessed by MTT method. The morphology of BMDCs stimulated with LPS remained immature after RAPA pretreatment. RAPA significantly decreased the CD86 expression, impaired the IL-12 and IFN-gamma production of BMDCs stimulated with LPS, and inhibited the proliferation of allogeneic T cells. In conclusion, RAPA can inhibit the maturation of BMDCs stimulated with LPS in terms of the morphology, surface phenotype, cytokine production, and ability of BMDCs to stimulate the proliferation of allogeneic T cells in vitro. |
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| Keywords: | rat dendritic cells rapamycin |
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