原发性高血压患者GNB3基因多态性的研究

目的探讨GNB3基因多态性与原发性高血压（EH）发病的关系。方法采用聚合酶链反应结合限制性内切酶片段长度多态分析方法（PCR-RFLP）对110例40岁及以上成年人和92例EH患者GNB3基因多态性进行检测,记录性别、年龄、糖尿病史、吸烟史、饮酒史,并测定体质量指数（BMI）、腰臀比（WHR）、总胆固醇（TC）、甘油三酯（TG）、高密度脂蛋白胆固醇（HDL-C）及空腹血糖（FPG）浓度。结果 C825T多态性位点在两组人群中的分布均符合Hardy-weinberg遗传平衡定律,EH患者中CC、CT、TT 3种基因型频率分布依次为32.2%、54.3%、13.0%;对照组3种基因型频率分布依次为30.9%、63.0%、5.5%;两组人群中825T等位基因频率分别为40.2%、37.3%,两组比较差异无统计学意义。结论 GNB3基因多态性可能与EH发病无关。

Association between gene polymorphism of GNB3 and essential hypertensive

Objective To identify the association between the C825T polymorphism of G protein β3 subunit with essential hypertension（EH）.Methods The C825T polymorphism of G protein β3 subunit was determined by polymerase chain reaction combined with restriction enzyme digestion（PCR-RFLP） in 110 normal controls over aged 40 and 92 cases of EH,including sex,age,diabetes mellitus history,smoking history,alcohol history,body mass index（BMI）,waist-hip ratio（WHR）,total cholesterol（TC）,triglyceride（TG）,high density lipoprotein cholesterol（HDL-C）,fasting plasma glucose（FPG）.Results C825T polymorphism was in agreement with Hardy-Weinberg equilibrium in two groups.The genotype frequencies of CC,CT and TT in EH were 32.2%,54.3%,13.0%;and those in control group were 30.9%,63.0%,5.5%.In addition,the 825T allele frequency was 40.2% in EH,and 37.3% in control group.Two groups had similar distribution in both genotype and allele frequency.There were no significant differences in two groups.Conclusion The C825T polymorphism of G protein β3 subunit was not associated with EH.