水蛭素冻干粉对大鼠的致畸性研究

目的：检测水蛭素冻干粉的致畸性。方法：将60只妊娠大鼠随机分为水蛭素冻干粉（每克含3mg、相当于50抗凝血酶单位的天然水蛭素）312.5、1250、5000mg/kg 3个剂量组,同时设蒸馏水阴性对照和阿司匹林（300mg/kg）阳性对照组,共5组,每组12只。各组孕鼠于妊娠第6～15天灌胃给予受试物,每天1次,连续灌胃10d,于妊娠第20天解剖孕鼠,检查胎鼠外观、内脏和骨骼及生长发育等指标。结果：水蛭素冻干粉各剂量组的孕鼠体质量、窝质量、胎鼠体质量、身长、尾长、活胎率、吸收胎率及死胎率与阴性对照组比较,差异均无统计学意义（P〉0.05）,未见胎鼠外观、内脏和骨骼发育异常及畸形。结论：水蛭素冻干粉在本实验条件下,对大鼠无母体毒性、胚胎毒性和致畸性。

Teratogenicity of lyophilized hirudin powder in rats

OBJECTIVE:To determine the potentially teratogenic effects of lyophilized hirudin powder in rats. METHODS:Sixty pregnant rats were divided randomly into 5 groups,3 groups treated with lyophilized hirudin powder (contain 3 mg which was equivalent to 50 AT-U natural hirudin per gram)with doses of 312.5,1250,5000 mg/kg,one negative control(distilled water)and a positive control(300 mg/kg aspirin),12 pregnant rats in each group.The drugs were given orally for 10 days from 6~(th)to 15~(th)day after gestation.At ...