258例血清中抗-HCV阳性联合HCV-RNA定量检测结果分析

目的探讨丙型肝炎病毒抗体（抗-HCV）和丙型肝炎病毒核糖核酸（HCV-RNA）定量联合检测结果对丙型肝炎确诊的临床意义。方法对258例ELISA法检测的血清抗-HCV阳性丙型肝炎（HC）患者,同时采用PCR-荧光探针法检测其HCV-RNA含量。结果 258例抗-HCV阳性丙型肝炎患者中HCV-RNA阴性92例,占35.66%;17例HCV-RNA弱阳性,占6.59%;HCV-RNA阳性149例,即57.75%的HC患者存在病毒血症。结论 ELISA法联合检测抗-HCV和HCV-RNA有助于丙型肝炎确诊病毒血症以及监控HCV的感染,并及时进行抗病毒治疗。

Result analysis of serum anti-HCV positive combined with HCV-RNA quantitative detection of 258 cases

Objective To investigate the clinical significance of hepatitis C virus antibody （anti-HCV） combined with hepatitis C virus ribonucleic acid （HCV-RNA） quantitative detection in the diagnosis of hepatitis C （HC）. Methods A total of 258 cases of HC with anti-HCV positive were detected by ELISA method and PCR- fluorescence probe for the HCV-RNA content. Results Among the 258 cases with anti-HCV positive, there were 92 cases of HCV-RNA negative as 35.66%, 17 cases of HCV-RNA weakly positive as 6.59%, and 149 cases of HCV-RNA positive. Thus, there were 57.75% of the HC patients with viremia. Conclusion ELISA method in combined detection of anti-HCV and HCV-RNA helps to diagnose viremia of HC, monitor HCV infection and conduct timely antiviral treatment.